Abstract
A gene expression study of Haemophilus ducreyi identified the hypothetical lipoprotein HD0192, renamed here "fibrinogen binder A" (FgbA), as being preferentially expressed in vivo. To test the role played by fgbA in virulence, an isogenic fgbA mutant (35000HPfgbA) was constructed using H. ducreyi 35000HP, and 6 volunteers were experimentally infected with 35000HP or 35000HPfgbA. The overall pustule-formation rate was 61.1% at parent sites and 22.2% at mutant sites (P = .019). Papules were significantly smaller at mutant sites than at parent sites (13.3 vs. 37.9 mm2; P = .002) 24 h after inoculation. Thus, fgbA contributed significantly to the virulence of H. ducreyi in humans. In vitro experiments demonstrated that fgbA encodes a fibrinogen-binding protein; no other fibrinogen-binding proteins were identified in 35000HP. fgbA was conserved among clinical isolates of both class I and II H. ducreyi strains, supporting the finding that fgbA is important for H. ducreyi infection.
| Original language | English |
|---|---|
| Pages (from-to) | 684-692 |
| Number of pages | 9 |
| Journal | Journal of Infectious Diseases |
| Volume | 199 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 1 2009 |
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ASJC Scopus subject areas
- Infectious Diseases
- Immunology and Allergy
Cite this
A fibrinogen-binding lipoprotein contributes to the virulence of Haemophilus ducreyi in humans. / Bauer, Margaret; Townsend, Carisa A.; Doster, Ryan S.; Fortney, Kate R.; Zwickl, Beth W.; Katz, Barry; Spinola, Stanley; Janowicz, Diane.
In: Journal of Infectious Diseases, Vol. 199, No. 5, 01.03.2009, p. 684-692.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A fibrinogen-binding lipoprotein contributes to the virulence of Haemophilus ducreyi in humans
AU - Bauer, Margaret
AU - Townsend, Carisa A.
AU - Doster, Ryan S.
AU - Fortney, Kate R.
AU - Zwickl, Beth W.
AU - Katz, Barry
AU - Spinola, Stanley
AU - Janowicz, Diane
PY - 2009/3/1
Y1 - 2009/3/1
N2 - A gene expression study of Haemophilus ducreyi identified the hypothetical lipoprotein HD0192, renamed here "fibrinogen binder A" (FgbA), as being preferentially expressed in vivo. To test the role played by fgbA in virulence, an isogenic fgbA mutant (35000HPfgbA) was constructed using H. ducreyi 35000HP, and 6 volunteers were experimentally infected with 35000HP or 35000HPfgbA. The overall pustule-formation rate was 61.1% at parent sites and 22.2% at mutant sites (P = .019). Papules were significantly smaller at mutant sites than at parent sites (13.3 vs. 37.9 mm2; P = .002) 24 h after inoculation. Thus, fgbA contributed significantly to the virulence of H. ducreyi in humans. In vitro experiments demonstrated that fgbA encodes a fibrinogen-binding protein; no other fibrinogen-binding proteins were identified in 35000HP. fgbA was conserved among clinical isolates of both class I and II H. ducreyi strains, supporting the finding that fgbA is important for H. ducreyi infection.
AB - A gene expression study of Haemophilus ducreyi identified the hypothetical lipoprotein HD0192, renamed here "fibrinogen binder A" (FgbA), as being preferentially expressed in vivo. To test the role played by fgbA in virulence, an isogenic fgbA mutant (35000HPfgbA) was constructed using H. ducreyi 35000HP, and 6 volunteers were experimentally infected with 35000HP or 35000HPfgbA. The overall pustule-formation rate was 61.1% at parent sites and 22.2% at mutant sites (P = .019). Papules were significantly smaller at mutant sites than at parent sites (13.3 vs. 37.9 mm2; P = .002) 24 h after inoculation. Thus, fgbA contributed significantly to the virulence of H. ducreyi in humans. In vitro experiments demonstrated that fgbA encodes a fibrinogen-binding protein; no other fibrinogen-binding proteins were identified in 35000HP. fgbA was conserved among clinical isolates of both class I and II H. ducreyi strains, supporting the finding that fgbA is important for H. ducreyi infection.
UR - http://www.scopus.com/inward/record.url?scp=61849155746&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61849155746&partnerID=8YFLogxK
U2 - 10.1086/596656
DO - 10.1086/596656
M3 - Article
C2 - 19199547
AN - SCOPUS:61849155746
VL - 199
SP - 684
EP - 692
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -