Ein retinoidbestandteil von lipofuszin, A2-E, ist ein photosensitizer in humanen RPE-Zellen

Translated title of the contribution: A fluorescent compound of lipofuscin, A2-E, is a photosensitizer in human retinal pigment epithelial cells

F. Schütt, S. Davies, J. Kopitz, M. Boulton, F. G. Holz

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background. A fluorescent compound of lipofuscin, A2-E, has been shown to impair lysosomal function and to increase the intraysosomal pH of human retinal pigment epithelial (RPE) cells. This study addressed the phototoxic potential of A2-E on RPE cells. Methods. A2-E accumulation was confirmed by fluorescence microscopy and fluorescence-activated cell sorter analysis. Acridine orange staining allowed assessment of lysosomal integrity and intralysosomal pH. Phototoxic properties of A2-E were determined by exposing A2-E-free and A2-E-fed RPE cell cultures to shortwavelength visible light and assessing cell viability and lysosomal integrity. Results. Intralysosomal accumulation of A2-E was confirmed. Acridine orange staining showed that the A2-E was located in the lysosomal compartment and induced an elevation of inralysosomal pH. Exposure of A2-E fed cells to light resulted in a significant loss of cell viability by 72 h which was not observed in either RPE cells maintained in the dark or A2-E-free cultures exposed to light. Toxicity was associated with a loss of lysosomal integrity. Conclusion. A2-E is detrimental to RPE cell function by a variety of mechanisms including inhibition of lysosomal degrading capacity, loss of membrane integrity, and phototoxity. Such mechanisms could contribute to retinal aging and to retinal diseases associated with excessive lipofuscin accumulation.

Original languageGerman
Pages (from-to)682-687
Number of pages6
JournalOphthalmologe
Volume97
Issue number10
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Lipofuscin
Photosensitizing Agents
Retinal Pigments
varespladib methyl
Epithelial Cells
Acridine Orange
Light
Cell Survival
A2-E (N-retinylidene-N-retinylethanolamine)
Staining and Labeling
Retinal Diseases
Fluorescence Microscopy
Cell Culture Techniques
Fluorescence

Keywords

  • A2-E
  • Age-related macular degeneration
  • Photosensitizer
  • Retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ein retinoidbestandteil von lipofuszin, A2-E, ist ein photosensitizer in humanen RPE-Zellen. / Schütt, F.; Davies, S.; Kopitz, J.; Boulton, M.; Holz, F. G.

In: Ophthalmologe, Vol. 97, No. 10, 2000, p. 682-687.

Research output: Contribution to journalArticle

Schütt, F, Davies, S, Kopitz, J, Boulton, M & Holz, FG 2000, 'Ein retinoidbestandteil von lipofuszin, A2-E, ist ein photosensitizer in humanen RPE-Zellen', Ophthalmologe, vol. 97, no. 10, pp. 682-687. https://doi.org/10.1007/s003470070037
Schütt, F. ; Davies, S. ; Kopitz, J. ; Boulton, M. ; Holz, F. G. / Ein retinoidbestandteil von lipofuszin, A2-E, ist ein photosensitizer in humanen RPE-Zellen. In: Ophthalmologe. 2000 ; Vol. 97, No. 10. pp. 682-687.
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