A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells

R. C. Ziegelstein, H. A. Spurgeon, Roberto Pili, A. Passaniti, L. Cheng, S. Corda, E. G. Lakatta, M. C. Capogrossi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The presence of the ryanodine receptor was recently demonstrated in vascular and endocardial endothelium, but its function has not been established. We investigated whether functional ryanodine-sensitive Ca2+ stores are present in cultured endothelial cells from rat aorta (RAECs), human aorta (HAECs), human umbilical vein (HUVECs), and bovine pulmonary artery (BPAECs) and what role these may play in intracellular Ca2+ regulation. Under resting conditions, HAECs, BPAECs, and HUVECs demonstrated a slow increase in intracellular Ca2+ (indexed by indo 1 fluorescence) on exposure to 5 μmol/L ryanodine, whereas RAECs did not. However, after an initial bradykinin exposure in RAECs, ryanodine markedly blunted the rapid increase in Ca2+ on a second exposure to bradykinin. In HUVECs, ryanodine in buffer with 1.5 mmol/L Ca2+ did not inhibit the agonist-sensitive Ca2+ increase, whereas it blunted the rapid increase in Ca2+ on histamine exposure in buffer with 5 mmol/L Ca2+, suggesting that increasing [Ca2+] enhances the binding of ryanodine to its receptor. Thus, functional ryanodine-sensitive Ca2+ stores are present in vascular endothelial cells. These appear to be involved in regulation of Ca2+ storage and release from agonist-sensitive intracellular compartments.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalCirculation Research
Volume74
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

Ryanodine
Endothelial Cells
Bradykinin
Aorta
Buffers
Umbilical Veins
Ryanodine Receptor Calcium Release Channel
Vascular Endothelium
Histamine
Pulmonary Artery
Cultured Cells
Fluorescence

Keywords

  • Ca
  • endothelium
  • indo 1
  • ryanodine

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Ziegelstein, R. C., Spurgeon, H. A., Pili, R., Passaniti, A., Cheng, L., Corda, S., ... Capogrossi, M. C. (1994). A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells. Circulation Research, 74(1), 151-156.

A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells. / Ziegelstein, R. C.; Spurgeon, H. A.; Pili, Roberto; Passaniti, A.; Cheng, L.; Corda, S.; Lakatta, E. G.; Capogrossi, M. C.

In: Circulation Research, Vol. 74, No. 1, 1994, p. 151-156.

Research output: Contribution to journalArticle

Ziegelstein, RC, Spurgeon, HA, Pili, R, Passaniti, A, Cheng, L, Corda, S, Lakatta, EG & Capogrossi, MC 1994, 'A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells', Circulation Research, vol. 74, no. 1, pp. 151-156.
Ziegelstein RC, Spurgeon HA, Pili R, Passaniti A, Cheng L, Corda S et al. A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells. Circulation Research. 1994;74(1):151-156.
Ziegelstein, R. C. ; Spurgeon, H. A. ; Pili, Roberto ; Passaniti, A. ; Cheng, L. ; Corda, S. ; Lakatta, E. G. ; Capogrossi, M. C. / A functional ryanodine-sensitive intracellular Ca2+ store is present in vascular endothelial cells. In: Circulation Research. 1994 ; Vol. 74, No. 1. pp. 151-156.
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