A GCN2-Like eukaryotic initiation factor 2 kinase increases the viability of extracellular toxoplasma gondii parasites

Christian Konrad, Ronald Wek, William Sullivan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Toxoplasmosis is a significant opportunistic infection caused by the protozoan parasite Toxoplasma gondii, an obligate intracellular pathogen that relies on host cell nutrients for parasite proliferation. Toxoplasma parasites divide until they rupture the host cell, at which point the extracellular parasites must survive until they find a new host cell. Recent studies have indicated that phosphorylation of Toxoplasma eukaryotic translation initiation factor 2-alpha (TgIF2α) plays a key role in promoting parasite viability during times of extracellular stress. Here we report the cloning and characterization of a TgIF2 α kinase designated TgIF2K-D that is related to GCN2, a eukaryotic initiation factor 2 α (eIF2 α) kinase known to respond to nutrient starvation in other organisms. TgIF2K-D is present in the cytosol of both intra- and extracellular Toxoplasma parasites and facilitates translational control through TgIF2 α phosphorylation in extracellular parasites. We generated a TgIF2K-D knockout parasite and demonstrated that loss of this eIF2 α kinase leads to a significant fitness defect that stems from an inability of the parasite to adequately adapt to the environment outside host cells. This phenotype is consistent with that reported for our nonphosphorylatable TgIF2 α mutant (S71A substitution), establishing that TgIF2K-D is the primary eIF2α kinase responsible for promoting extracellular viability of Toxoplasma. These studies suggest that eIF2α phosphorylation and translational control are an important mechanism by which vulnerable extracellular parasites protect themselves while searching for a new host cell. Additionally, TgIF2α is phosphorylated when intracellular parasites are deprived of nutrients, but this can occur independently of TgIF2K-D, indicating that this activity can be mediated by a different TgIF2K.

Original languageEnglish
Pages (from-to)1403-1412
Number of pages10
JournalEukaryotic Cell
Volume10
Issue number11
DOIs
StatePublished - Nov 2011

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Eukaryotic Initiation Factor-2
Toxoplasma
Parasites
Phosphotransferases
Phosphorylation
Food
Eukaryotic Initiation Factors
Toxoplasmosis
Opportunistic Infections
Starvation
Cytosol
Organism Cloning
Rupture

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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A GCN2-Like eukaryotic initiation factor 2 kinase increases the viability of extracellular toxoplasma gondii parasites. / Konrad, Christian; Wek, Ronald; Sullivan, William.

In: Eukaryotic Cell, Vol. 10, No. 11, 11.2011, p. 1403-1412.

Research output: Contribution to journalArticle

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