A label free quantitative proteomic analysis of the Saccharomyces cerevisiae nucleus

Amber L. Mosley, Laurence Florens, Zhihui Wen, Michael P. Washburn

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

To gain insight into the nuclear proteome of Saccharomyces cerevisiae, nuclei were isolated and fractionated via sucrose gradient sedimentation. The resulting fractions were analyzed using multidimensional protein identification technology and the detected proteins were quantified using normalized spectral counts. A large number of low abundance proteins, many of which are involved in transcriptional regulation, were recovered. Sucrose gradient elution profiles of known protein complex components demonstrated that this approach may provide insight into the question of what percentage of the total population of a protein is in one complex, versus another protein complex, or exists as a free protein.

Original languageEnglish (US)
Pages (from-to)110-120
Number of pages11
JournalJournal of Proteomics
Volume72
Issue number1
DOIs
StatePublished - Feb 15 2009

Keywords

  • Multidimensional protein identification technology
  • Normalized spectralabundance factor
  • Nucleus
  • Protein complexes
  • Quantitative proteomics
  • Transcription factors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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