A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension

Allen J. Naftilan, Roger Williams, David Burt, Martin Paul, Richard E. Pratt, Peter Hobart, John Chirgwin, Victor J. Dzau

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Because renin is an important enzyme in blood pressure regulation, we studied the possibility that an alteration in the structure of the human renin gene is genetically linked to human essential hypertension or associated with levels of plasma renin activity or blood pressure. By using specific DNA probes, we have identified four polymorphisms in the human renin gene with the restriction enzymes Taq I, HindIII, Bgl I, and Bgl II. The gene location of all of these polymorphisms except for the Bgl II polymorphism has been determined, and their frequencies were initially estimated in a population of 50 random subjects. To test the clinical significance of these polymorphisms, we studied 68 persons from a large Utah pedigree with a high incidence of hypertension. Among nine relatives with hypertension, genetic linkage without recombination was ruled out by observing several obligate recombinants. We also found no significant association of the restriction fragment length polymorphisms with quantitative measurements of sitting or standing, systolic or diastolic blood pressures, or plasma renin activity in 59 untreated members of this pedigree. Although we found no genetic linkage in this set of study subjects, the characterization of the restriction fragment length polymorphisms for the renin gene may be useful in future studies of other selected pedigrees for the presence of one or more of these to be a genetic marker in hypertension.

Original languageEnglish (US)
Pages (from-to)614-618
Number of pages5
JournalHypertension
Volume14
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Genetic Linkage
Renin
Restriction Fragment Length Polymorphisms
Hypertension
Pedigree
Genes
Blood Pressure
DNA Probes
Enzymes
Genetic Markers
Genetic Recombination
Incidence
Population

Keywords

  • Polymorphisms
  • Renin
  • Renin gene

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Naftilan, A. J., Williams, R., Burt, D., Paul, M., Pratt, R. E., Hobart, P., ... Dzau, V. J. (1989). A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension, 14(6), 614-618.

A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. / Naftilan, Allen J.; Williams, Roger; Burt, David; Paul, Martin; Pratt, Richard E.; Hobart, Peter; Chirgwin, John; Dzau, Victor J.

In: Hypertension, Vol. 14, No. 6, 1989, p. 614-618.

Research output: Contribution to journalArticle

Naftilan, AJ, Williams, R, Burt, D, Paul, M, Pratt, RE, Hobart, P, Chirgwin, J & Dzau, VJ 1989, 'A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension', Hypertension, vol. 14, no. 6, pp. 614-618.
Naftilan AJ, Williams R, Burt D, Paul M, Pratt RE, Hobart P et al. A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. Hypertension. 1989;14(6):614-618.
Naftilan, Allen J. ; Williams, Roger ; Burt, David ; Paul, Martin ; Pratt, Richard E. ; Hobart, Peter ; Chirgwin, John ; Dzau, Victor J. / A lack of genetic linkage of renin gene restriction fragment length polymorphisms with human hypertension. In: Hypertension. 1989 ; Vol. 14, No. 6. pp. 614-618.
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