A liquid chromatography/tandem mass spectrometry assay to quantitate MS-275 in human plasma

Ming Zhao, Michelle A. Rudek, Aleksandr Mnasakanyan, Carol Hartke, Roberto Pili, Sharyn D. Baker

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A rapid, sensitive and selective method was developed and validated using LC/MS/MS for determination of MS-275 in human plasma. Sample preparation involved a single step liquid-liquid extraction by the addition of 0.2 ml of plasma with 5 ml acetonitrile/n-butyl-chloride. Separation of the compounds of interest, including the internal standard paclitaxel, was achieved on a Waters X-Terra™ C18 (50 mm × 2.1 mm i.d., 3.5 μm) analytical column using a mobile phase consisting of acetonitrile/ammonium acetate (pH 2.9; 2 mM)(60:40, v/v) containing 0.1% formic acid and isocratic flow at 0.15 ml/min for 3 min. The analytes were monitored by tandem-mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 0.5-100 ng/ml with values for the coefficient of determination of >0.99. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods (<15%). This method was subsequently used to measure concentrations of MS-275 in cancer patients receiving an oral weekly dose of 4 mg/m2.

Original languageEnglish (US)
Pages (from-to)784-787
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume43
Issue number2
DOIs
StatePublished - Jan 17 2007
Externally publishedYes

Keywords

  • LC/MS/MS
  • MS-275
  • Pharmacokinetics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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