A method for processing free-floating cultured cells for scanning electron microscopy

James A. McAteer, Andrew P. Evan, William H.J. Douglas

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A simple and convenient method is described to aid the processing of minute tissue specimens and isolated single cells for scanning electron microscopy (SEM). The technique employs an inexpensive, disposable carrier fashioned from common Beem capsules and Nitex nylon screen. This method avoids excessive handling, transfers or centrifugation steps that may damage delicate specimens. Specimens processed by this method show excellent preservation of cell surface fine structure.

Original languageEnglish (US)
Pages (from-to)85-88
Number of pages4
JournalJournal of Tissue Culture Methods
Volume7
Issue number3
DOIs
StatePublished - Sep 1982

Keywords

  • aggregate cultures
  • isolated cells
  • scanning electron microscopy
  • specimen processing

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

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