A method for quantification of astrocytic processes reveals laminar differences in the dentate gyms but not in CA1 of the hippocampal formation

Krystyna R. Isaacs, Anita M. Sirevaag, Amy E. Marks, Fen-Lei Chang, William T. Greenough

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The distribution of astrocytic processes in the dentate gyrus molecular layer and CA1 subregion of the hippocampal formation was examined in 8-μm frozen sections from adult male rats. The stereological cycloid intersection method (Baddeley et al., 1986) was used to estimate surface density of glial fibrillary acidic protein-immunoreactive astrocytic processes. A distinct lamination pattern was delineated in the dentate gyrus which corresponds to the afferent input to this region. No such pattern was detectable in s. radiatum of CA1. By using this relatively new stereological method which utilizes a sensitive sampling scheme, it was possible to increase the reliability in reporting the distribution of fine processes while at the same time greatly reducing the amount of time necessary to estimate the amount of astrocytic processes in a given region.

Original languageEnglish (US)
Pages (from-to)141-148
Number of pages8
JournalJournal of Neuroscience Methods
Volume48
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

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Dentate Gyrus
Hippocampus
Glial Fibrillary Acidic Protein
Frozen Sections

Keywords

  • GFAP
  • Glia
  • Hippocampus
  • Stereology

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A method for quantification of astrocytic processes reveals laminar differences in the dentate gyms but not in CA1 of the hippocampal formation. / Isaacs, Krystyna R.; Sirevaag, Anita M.; Marks, Amy E.; Chang, Fen-Lei; Greenough, William T.

In: Journal of Neuroscience Methods, Vol. 48, No. 1-2, 1993, p. 141-148.

Research output: Contribution to journalArticle

Isaacs, Krystyna R. ; Sirevaag, Anita M. ; Marks, Amy E. ; Chang, Fen-Lei ; Greenough, William T. / A method for quantification of astrocytic processes reveals laminar differences in the dentate gyms but not in CA1 of the hippocampal formation. In: Journal of Neuroscience Methods. 1993 ; Vol. 48, No. 1-2. pp. 141-148.
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