A model for CD8+ CTL tumor immunosurveillance and regulation of tumor escape by CD4 T cells through an effect on quality of CTL

So Matsui, Jeffrey D. Ahlers, Alexander Vortmeyer, Masaki Terabe, Taku Tsukui, David P. Carbone, Lance A. Liotta, Jay A. Berzofsky

Research output: Contribution to journalArticle

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Abstract

Understanding immune mechanisms influencing cancer regression, recurrence, and metastasis may be critical to developing effective immunotherapy. Using a tumor expressing HIV gp160 as a model viral tumor Ag, we found a growth-regression-recurrence pattern, and used this to investigate mechanisms of immunosurveillance. Regression was dependent on CD8 T cells, and recurrent tumors were resistant to CTL, had substantially reduced expression of epitope mRNA, but retained the gp160 gene, MHC, and processing apparatus. Increasing CTL numbers by advance priming with vaccinia virus expressing gp160 prevented only the initial tumor growth but not the later appearance of escape variants. Unexpectedly, CD4 cell depletion protected mice from tumor recurrence, whereas IL-4 knockout mice, deficient in Th2 cells, did not show this protection, and IFN-γ knockout mice were more susceptible. Purified CD8 T cells from CD4-depleted mice following tumor regression had more IFN-γ mRNA and lysed tumor cells without stimulation ex vivo, in contrast to CD4-intact mice. Thus, the quality as well as quantity of CD8+ CTL determines the completeness of immunosurveillance and is controlled by CD4 T cells but not solely Th2 cytokines. This model of immunosurveillance may indicate ways to enhance the efficacy of surveillance and improve immunotherapy.

Original languageEnglish (US)
Pages (from-to)184-193
Number of pages10
JournalJournal of Immunology
Volume163
Issue number1
StatePublished - Jul 1 1999
Externally publishedYes

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Tumor Escape
Immunologic Monitoring
T-Lymphocytes
Neoplasms
Knockout Mice
Recurrence
Immunotherapy
HIV Envelope Protein gp160
Th2 Cells
Messenger RNA
Vaccinia virus
Growth
Interleukin-4
Epitopes
Cytokines
Neoplasm Metastasis

ASJC Scopus subject areas

  • Immunology

Cite this

Matsui, S., Ahlers, J. D., Vortmeyer, A., Terabe, M., Tsukui, T., Carbone, D. P., ... Berzofsky, J. A. (1999). A model for CD8+ CTL tumor immunosurveillance and regulation of tumor escape by CD4 T cells through an effect on quality of CTL. Journal of Immunology, 163(1), 184-193.

A model for CD8+ CTL tumor immunosurveillance and regulation of tumor escape by CD4 T cells through an effect on quality of CTL. / Matsui, So; Ahlers, Jeffrey D.; Vortmeyer, Alexander; Terabe, Masaki; Tsukui, Taku; Carbone, David P.; Liotta, Lance A.; Berzofsky, Jay A.

In: Journal of Immunology, Vol. 163, No. 1, 01.07.1999, p. 184-193.

Research output: Contribution to journalArticle

Matsui, S, Ahlers, JD, Vortmeyer, A, Terabe, M, Tsukui, T, Carbone, DP, Liotta, LA & Berzofsky, JA 1999, 'A model for CD8+ CTL tumor immunosurveillance and regulation of tumor escape by CD4 T cells through an effect on quality of CTL', Journal of Immunology, vol. 163, no. 1, pp. 184-193.
Matsui, So ; Ahlers, Jeffrey D. ; Vortmeyer, Alexander ; Terabe, Masaki ; Tsukui, Taku ; Carbone, David P. ; Liotta, Lance A. ; Berzofsky, Jay A. / A model for CD8+ CTL tumor immunosurveillance and regulation of tumor escape by CD4 T cells through an effect on quality of CTL. In: Journal of Immunology. 1999 ; Vol. 163, No. 1. pp. 184-193.
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