A model for fd phage penetration and assembly

A. Dunker, Lars D. Ensign, Gregory E. Arnold, Linda M. Roberts

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Below 15°C, chloroform causes fd phage to contract to I-forms, which are compact structures about 1/3 as long as the original phage. Above 15°C, chloroform causes I-forms to contract to even more compact spheroidal S-forms. Here we show that the coat protein structure in I-forms is the same as the protein structure in the phage and the protein structure in S-forms is the same as the protein structure in bilayers. The conversions from fdåI-forms→S-forms are therefore suggested to mimic steps in fd penetration. The same conversions, in reverse order, are suggested to mimic steps in fd assembly.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalFEBS Letters
Volume292
Issue number1-2
DOIs
StatePublished - Nov 4 1991
Externally publishedYes

Fingerprint

Bacteriophage M13
Bacteriophages
Chloroform
Proteins
Capsid Proteins

Keywords

  • Filamentous phage assembly

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Dunker, A., Ensign, L. D., Arnold, G. E., & Roberts, L. M. (1991). A model for fd phage penetration and assembly. FEBS Letters, 292(1-2), 271-274. https://doi.org/10.1016/0014-5793(91)80882-4

A model for fd phage penetration and assembly. / Dunker, A.; Ensign, Lars D.; Arnold, Gregory E.; Roberts, Linda M.

In: FEBS Letters, Vol. 292, No. 1-2, 04.11.1991, p. 271-274.

Research output: Contribution to journalArticle

Dunker, A, Ensign, LD, Arnold, GE & Roberts, LM 1991, 'A model for fd phage penetration and assembly', FEBS Letters, vol. 292, no. 1-2, pp. 271-274. https://doi.org/10.1016/0014-5793(91)80882-4
Dunker, A. ; Ensign, Lars D. ; Arnold, Gregory E. ; Roberts, Linda M. / A model for fd phage penetration and assembly. In: FEBS Letters. 1991 ; Vol. 292, No. 1-2. pp. 271-274.
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