A model for fd phage penetration and assembly

A. Keith Dunker, Lars D. Ensign, Gregory E. Arnold, Linda M. Roberts

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Below 15°C, chloroform causes fd phage to contract to I-forms, which are compact structures about 1/3 as long as the original phage. Above 15°C, chloroform causes I-forms to contract to even more compact spheroidal S-forms. Here we show that the coat protein structure in I-forms is the same as the protein structure in the phage and the protein structure in S-forms is the same as the protein structure in bilayers. The conversions from fdåI-forms→S-forms are therefore suggested to mimic steps in fd penetration. The same conversions, in reverse order, are suggested to mimic steps in fd assembly.

Original languageEnglish (US)
Pages (from-to)271-274
Number of pages4
JournalFEBS Letters
Volume292
Issue number1-2
DOIs
StatePublished - Nov 4 1991

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Keywords

  • Filamentous phage assembly

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dunker, A. K., Ensign, L. D., Arnold, G. E., & Roberts, L. M. (1991). A model for fd phage penetration and assembly. FEBS Letters, 292(1-2), 271-274. https://doi.org/10.1016/0014-5793(91)80882-4