A native soluble form of CTLA-4

Martin K. Oaks, Karen M. Hallett, R. Todd Penwell, Edward C. Stauber, Simon Warren, Alfred J. Tector

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

CTLA-4 is an immunoregulatory receptor expressed on the surface of activated T and B lymphocytes. The counterreceptors for CTLA-4 are the B7 family molecules. We describe alternatively spliced mRNAs expressed in hematolymphoid tissues of humans, mice, and rats that lack the transmembrane domain coded by exon 3 of the CTLA-4 gene. These alternate transcripts were detected by RT-PCR in B cells and resting T cells of both the CD4 and the CD8 phenotype. Activation of human blood mononuclear cells with PHA or anti-CD3 + anti-CD28 monoclonal antibodies appears to effect a decrease in the amount of the alternative transcript relative to the full-length transcript. Recombinant sCTLA-4 is a B7-binding protein and has immunomodulatory effects as measured by inhibition of the mixed leukocyte response. Human serum contains immunoreactive material consistent with a native soluble form of CTLA-4. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)144-153
Number of pages10
JournalCellular Immunology
Volume201
Issue number2
DOIs
StatePublished - May 1 2000
Externally publishedYes

Fingerprint

B-Lymphocytes
B7 Antigens
T-Lymphocytes
Exons
Blood Cells
Carrier Proteins
Leukocytes
Monoclonal Antibodies
Phenotype
Polymerase Chain Reaction
Messenger RNA
Serum
Genes

Keywords

  • Comparative immunology
  • MRNA
  • T lymphocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Oaks, M. K., Hallett, K. M., Penwell, R. T., Stauber, E. C., Warren, S., & Tector, A. J. (2000). A native soluble form of CTLA-4. Cellular Immunology, 201(2), 144-153. https://doi.org/10.1006/cimm.2000.1649

A native soluble form of CTLA-4. / Oaks, Martin K.; Hallett, Karen M.; Penwell, R. Todd; Stauber, Edward C.; Warren, Simon; Tector, Alfred J.

In: Cellular Immunology, Vol. 201, No. 2, 01.05.2000, p. 144-153.

Research output: Contribution to journalArticle

Oaks, MK, Hallett, KM, Penwell, RT, Stauber, EC, Warren, S & Tector, AJ 2000, 'A native soluble form of CTLA-4', Cellular Immunology, vol. 201, no. 2, pp. 144-153. https://doi.org/10.1006/cimm.2000.1649
Oaks MK, Hallett KM, Penwell RT, Stauber EC, Warren S, Tector AJ. A native soluble form of CTLA-4. Cellular Immunology. 2000 May 1;201(2):144-153. https://doi.org/10.1006/cimm.2000.1649
Oaks, Martin K. ; Hallett, Karen M. ; Penwell, R. Todd ; Stauber, Edward C. ; Warren, Simon ; Tector, Alfred J. / A native soluble form of CTLA-4. In: Cellular Immunology. 2000 ; Vol. 201, No. 2. pp. 144-153.
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