A native soluble form of CTLA-4

Martin K. Oaks, Karen M. Hallett, R. Todd Penwell, Edward C. Stauber, Simon J. Warren, Alfred J. Tector

Research output: Contribution to journalArticle

188 Scopus citations

Abstract

CTLA-4 is an immunoregulatory receptor expressed on the surface of activated T and B lymphocytes. The counterreceptors for CTLA-4 are the B7 family molecules. We describe alternatively spliced mRNAs expressed in hematolymphoid tissues of humans, mice, and rats that lack the transmembrane domain coded by exon 3 of the CTLA-4 gene. These alternate transcripts were detected by RT-PCR in B cells and resting T cells of both the CD4 and the CD8 phenotype. Activation of human blood mononuclear cells with PHA or anti-CD3 + anti-CD28 monoclonal antibodies appears to effect a decrease in the amount of the alternative transcript relative to the full-length transcript. Recombinant sCTLA-4 is a B7-binding protein and has immunomodulatory effects as measured by inhibition of the mixed leukocyte response. Human serum contains immunoreactive material consistent with a native soluble form of CTLA-4.

Original languageEnglish (US)
Pages (from-to)144-153
Number of pages10
JournalCellular Immunology
Volume201
Issue number2
DOIs
StatePublished - May 1 2000

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Keywords

  • Comparative immunology
  • MRNA
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Oaks, M. K., Hallett, K. M., Penwell, R. T., Stauber, E. C., Warren, S. J., & Tector, A. J. (2000). A native soluble form of CTLA-4. Cellular Immunology, 201(2), 144-153. https://doi.org/10.1006/cimm.2000.1649