A negative regulatory element upstream from the mouse Adh-1 gene can down-regulate a heterologous promoter

Mang Yu, Ronald E. Jerome, Howard J. Edenberg

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Regulation of Adh-1 (encoding mouse alcohol dehydrogenase-A2) gene expression results from the interplay of positive and negative effects. We report here the detection and localization of a negative regulatory element (NRE) to the sequence between -754 and -732 bp relative to the transcription start point (tsp). This element, which we call Adh-1 NRE2, can down-regulate transcription from a strong heterologous promoter (the SV40 promoter + enhancer). Adh-1 NRE2 can function in both orientations and either upstream or downstream from the promoter, and thus has attributes characteristic of a 'silencer'. A protein that binds to the Adh-1 NRE is widespread in mouse tissues.

Original languageEnglish (US)
Pages (from-to)249-254
Number of pages6
JournalGene
Volume141
Issue number2
DOIs
StatePublished - Apr 20 1994

Keywords

  • alcohol dehydrogenase
  • cis-acting elements
  • DNase footprinting
  • gel mobility shift assay
  • Gene expression
  • silencer

ASJC Scopus subject areas

  • Genetics

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