A new natriuretic peptide isolated from cardiac atria of trout, Oncorhynchus mykiss

Y. Takei, A. Fukuzawa, Y. Itahara, T. X. Watanabe, K. Yoshizawa Kumagaye, K. Nakajima, A. Yasuda, M. P. Smith, D. W. Duff, K. R. Olson

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Atrial and brain natriuretic peptides (ANP and BNP, respectively) are two cardiac natriuretic peptides (NPs) found in tetrapods from amphibians to mammals; whereas ANP and ventricular NP (VNP) have been identified in eel hearts. Because VNP has also been found in the rainbow trout ventricle, we attempted to isolate NP from trout cardiac atria in order to determine whether ANP and VNP are common cardiac NPs in teleosts. In the present experiments, we isolated VNP and a novel atrial NP consisting of 29 amino acid residues from the atria. This new trout NP exhibited similar sequence identity to mammalian ANP and BNP (50-60%). Its homology to eel ANP was low (52%) compared with high homology of trout and eel VNP (78%). Based on yield, the content of this new NP in trout atria may be even smaller than that of VNP. The new trout atrial NP exhibited low relaxant activity in the chick rectum (only 1/10 of that of trout VNP), and extremely low vasorelaxant activity in the rat aortic strip (only 1/400 of that of human ANP). However, the new trout NP was equipotent with trout VNP and human ANP in relaxing trout epibranchial artery. Based on the sequence similarity with other NPs and on atrial content, the new NP isolated from trout atria cannot yet be assigned to a known member of the NP family.

Original languageEnglish (US)
Pages (from-to)377-380
Number of pages4
JournalFEBS Letters
Volume414
Issue number2
DOIs
StatePublished - Sep 8 1997

Keywords

  • Atrial natriuretic peptide
  • Brain natriuretic peptide
  • Molecular evolution
  • Rainbow trout (Oncorhynchus mykiss)
  • Vasorelaxant activity
  • Ventricular natriuretic peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Takei, Y., Fukuzawa, A., Itahara, Y., Watanabe, T. X., Kumagaye, K. Y., Nakajima, K., Yasuda, A., Smith, M. P., Duff, D. W., & Olson, K. R. (1997). A new natriuretic peptide isolated from cardiac atria of trout, Oncorhynchus mykiss. FEBS Letters, 414(2), 377-380. https://doi.org/10.1016/S0014-5793(97)01050-8