A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus

Peng R. Chen, Satoshi Nishida, Catherine B. Poor, Alice Cheng, Taeok Bae, Lisa Kuechenmeister, Paul M. Dunman, Dominique Missiakas, Chuan He

Research output: Contribution to journalArticle

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Abstract

Oxidative stress serves as an important host/environmental signal that triggers a wide range of responses from the human pathogen Staphylococcus aureus. Among these, a thiol-based oxidation sensing pathway through a global regulator MgrA controls the virulence and antibiotic resistance of the bacterium. Herein, we report a new thiol-based oxidation sensing and regulation system that is mediated through a parallel global regulator SarZ. SarZ is a functional homologue of MgrA and is shown to affect the expression of ∼87 genes in S. aureus. It uses a key Cys residue, Cys-13, to sense oxidative stress and to co-ordinate the expression of genes involved in metabolic switching, antibiotic resistance, peroxide stress defence, virulence, and cell wall properties. The discovery of this SarZ-mediated regulation, mostly independent from the MgrA-based regulation, fills a missing gap of oxidation sensing and response in S. aureus.

Original languageEnglish
Pages (from-to)198-211
Number of pages14
JournalMolecular Microbiology
Volume71
Issue number1
DOIs
StatePublished - Jan 2009

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Staphylococcus aureus
Microbial Drug Resistance
Sulfhydryl Compounds
Virulence
Oxidative Stress
Gene Expression
Peroxides
Cell Wall
Bacteria

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus. / Chen, Peng R.; Nishida, Satoshi; Poor, Catherine B.; Cheng, Alice; Bae, Taeok; Kuechenmeister, Lisa; Dunman, Paul M.; Missiakas, Dominique; He, Chuan.

In: Molecular Microbiology, Vol. 71, No. 1, 01.2009, p. 198-211.

Research output: Contribution to journalArticle

Chen, PR, Nishida, S, Poor, CB, Cheng, A, Bae, T, Kuechenmeister, L, Dunman, PM, Missiakas, D & He, C 2009, 'A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus', Molecular Microbiology, vol. 71, no. 1, pp. 198-211. https://doi.org/10.1111/j.1365-2958.2008.06518.x
Chen, Peng R. ; Nishida, Satoshi ; Poor, Catherine B. ; Cheng, Alice ; Bae, Taeok ; Kuechenmeister, Lisa ; Dunman, Paul M. ; Missiakas, Dominique ; He, Chuan. / A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus. In: Molecular Microbiology. 2009 ; Vol. 71, No. 1. pp. 198-211.
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