A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex

Ramadurgam Kodandapani, Frédéric Pio, Chao Zhou Ni, Gennaro Picciallit, Michael Klemsz, Scott McKercher, Richard A. Maki, Kathryn R. Ely

Research output: Contribution to journalArticle

248 Citations (Scopus)

Abstract

THE Ets family of transcription factors, of which there are now about 35 members1,2, regulate gene expression during growth and development. They share a conserved domain of around 85 amino acids3 which binds as a monomer to the DNA sequence 5′-C/ AGGAA/T-3′. We have determined the crystal structure of an ETS domain complexed with DNA, at 23-Å resolution. The domain is similar to α + β (winged) 'helix-turn-helix' proteins and interacts with a ten-base-pair region of duplex DNA which takes up a uniform curve of 8°. The domain contacts the DNA by a novel loop-helix-loop architecture. Four of the amino acids that directly interact with the DNA are highly conserved: two argi nines from the recognition helix lying in the major groove, one lysine from the 'wing' that binds upstream of the core GGAA sequence, and another lysine, from the 'turn' of the 'helix-turn-helix' motif, which binds downstream and on the opposite strand.

Original languageEnglish
Pages (from-to)456-460
Number of pages5
JournalNature
Volume380
Issue number6573
StatePublished - Apr 4 1996

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DNA
Lysine
Helix-Turn-Helix Motifs
Proto-Oncogene Proteins c-ets
Growth and Development
Base Pairing
Gene Expression
Amino Acids
ETS Motif
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kodandapani, R., Pio, F., Ni, C. Z., Picciallit, G., Klemsz, M., McKercher, S., ... Ely, K. R. (1996). A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex. Nature, 380(6573), 456-460.

A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex. / Kodandapani, Ramadurgam; Pio, Frédéric; Ni, Chao Zhou; Picciallit, Gennaro; Klemsz, Michael; McKercher, Scott; Maki, Richard A.; Ely, Kathryn R.

In: Nature, Vol. 380, No. 6573, 04.04.1996, p. 456-460.

Research output: Contribution to journalArticle

Kodandapani, R, Pio, F, Ni, CZ, Picciallit, G, Klemsz, M, McKercher, S, Maki, RA & Ely, KR 1996, 'A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex', Nature, vol. 380, no. 6573, pp. 456-460.
Kodandapani R, Pio F, Ni CZ, Picciallit G, Klemsz M, McKercher S et al. A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex. Nature. 1996 Apr 4;380(6573):456-460.
Kodandapani, Ramadurgam ; Pio, Frédéric ; Ni, Chao Zhou ; Picciallit, Gennaro ; Klemsz, Michael ; McKercher, Scott ; Maki, Richard A. ; Ely, Kathryn R. / A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex. In: Nature. 1996 ; Vol. 380, No. 6573. pp. 456-460.
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