A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine

J. W. Kolaczynski, L. M. Morales, J. H. Moore, Robert Considine, Z. Pietrzkowski, P. F. Noto, J. Colberg, J. F. Caro

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The objectives of this study were to develop a new technique to safely, reliably, and in a cosmetically acceptable way, obtain more than 5.0 g of abdominal subcutaneous fat in out-patients, and to investigate whether inhibitory effects of a local anaesthetic on adipose tissue function in vitro are sufficient argument against the use of infiltrative local anaesthesia during fat biopsy to obtain samples for metabolic studies. Measurements were obtained to compare glucose transport and lipolysis response to insulin in adipocytes isolated from subcutaneous abdominal fat obtained: (i) during elective surgery in eight women and four men (BMI 25.8 ± 3.1 kg/m2); and (ii) from five male and three female out-patients (BMI 25.8 ± 3.1 kg/m2) by the described novel technique performed under local anaesthesia with Lidocaine. The effects of acute and 11-day exposure to Lidocaine in vitro on adipocyte lipolysis and glucose transport response to insulin, and the growth potential were determined. In vivo exposure of the tissue samples to local anaesthetic by the novel technique had no apparent effect on isolated adipocyte responses to insulin by stimulation of glucose transport or by inhibitor- or adrenaline-stimulated lipolysis; the results were not different to those for adipocytes isolated from fat obtained during elective abdominal surgeries. Lidocaine added in vitro potently inhibited glucose transport and lipolysis in adipocytes, and cell attachment and growth in primary 'ceiling' culture, this effect persisted only as long as Lidocaine was present. After washing, adipocytes fully regained their function and growth regardless of the exposure period, as short as 30 min or as long as 11 days. Therefore, the proposed novel technique allows rapid sampling of significant amounts of abdominal fat in outpatients. Lidocaine has an inhibitory effect on adipocyte function; however, this effect appears to be fully reversible.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalInternational Journal of Obesity
Volume18
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Abdominal Fat
lidocaine
abdominal fat
Local Anesthesia
Lidocaine
adipocytes
Adipocytes
biopsy
anesthesia
Biopsy
Lipolysis
lipolysis
Abdominal Subcutaneous Fat
Glucose
local anesthetics
glucose
Outpatients
insulin
Insulin
Local Anesthetics

Keywords

  • Adipose tissue biopsy
  • Glucose transport
  • Lidocaine
  • Lipolysis

ASJC Scopus subject areas

  • Food Science
  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Public Health, Environmental and Occupational Health

Cite this

Kolaczynski, J. W., Morales, L. M., Moore, J. H., Considine, R., Pietrzkowski, Z., Noto, P. F., ... Caro, J. F. (1994). A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine. International Journal of Obesity, 18(3), 161-166.

A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine. / Kolaczynski, J. W.; Morales, L. M.; Moore, J. H.; Considine, Robert; Pietrzkowski, Z.; Noto, P. F.; Colberg, J.; Caro, J. F.

In: International Journal of Obesity, Vol. 18, No. 3, 1994, p. 161-166.

Research output: Contribution to journalArticle

Kolaczynski, JW, Morales, LM, Moore, JH, Considine, R, Pietrzkowski, Z, Noto, PF, Colberg, J & Caro, JF 1994, 'A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine', International Journal of Obesity, vol. 18, no. 3, pp. 161-166.
Kolaczynski JW, Morales LM, Moore JH, Considine R, Pietrzkowski Z, Noto PF et al. A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine. International Journal of Obesity. 1994;18(3):161-166.
Kolaczynski, J. W. ; Morales, L. M. ; Moore, J. H. ; Considine, Robert ; Pietrzkowski, Z. ; Noto, P. F. ; Colberg, J. ; Caro, J. F. / A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine. In: International Journal of Obesity. 1994 ; Vol. 18, No. 3. pp. 161-166.
@article{712109f5d1b848d89453f57bda0d4a55,
title = "A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine",
abstract = "The objectives of this study were to develop a new technique to safely, reliably, and in a cosmetically acceptable way, obtain more than 5.0 g of abdominal subcutaneous fat in out-patients, and to investigate whether inhibitory effects of a local anaesthetic on adipose tissue function in vitro are sufficient argument against the use of infiltrative local anaesthesia during fat biopsy to obtain samples for metabolic studies. Measurements were obtained to compare glucose transport and lipolysis response to insulin in adipocytes isolated from subcutaneous abdominal fat obtained: (i) during elective surgery in eight women and four men (BMI 25.8 ± 3.1 kg/m2); and (ii) from five male and three female out-patients (BMI 25.8 ± 3.1 kg/m2) by the described novel technique performed under local anaesthesia with Lidocaine. The effects of acute and 11-day exposure to Lidocaine in vitro on adipocyte lipolysis and glucose transport response to insulin, and the growth potential were determined. In vivo exposure of the tissue samples to local anaesthetic by the novel technique had no apparent effect on isolated adipocyte responses to insulin by stimulation of glucose transport or by inhibitor- or adrenaline-stimulated lipolysis; the results were not different to those for adipocytes isolated from fat obtained during elective abdominal surgeries. Lidocaine added in vitro potently inhibited glucose transport and lipolysis in adipocytes, and cell attachment and growth in primary 'ceiling' culture, this effect persisted only as long as Lidocaine was present. After washing, adipocytes fully regained their function and growth regardless of the exposure period, as short as 30 min or as long as 11 days. Therefore, the proposed novel technique allows rapid sampling of significant amounts of abdominal fat in outpatients. Lidocaine has an inhibitory effect on adipocyte function; however, this effect appears to be fully reversible.",
keywords = "Adipose tissue biopsy, Glucose transport, Lidocaine, Lipolysis",
author = "Kolaczynski, {J. W.} and Morales, {L. M.} and Moore, {J. H.} and Robert Considine and Z. Pietrzkowski and Noto, {P. F.} and J. Colberg and Caro, {J. F.}",
year = "1994",
language = "English (US)",
volume = "18",
pages = "161--166",
journal = "International Journal of Obesity",
issn = "0307-0565",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - A new technique for biopsy of human abdominal fat under local anaesthesia with Lidocaine

AU - Kolaczynski, J. W.

AU - Morales, L. M.

AU - Moore, J. H.

AU - Considine, Robert

AU - Pietrzkowski, Z.

AU - Noto, P. F.

AU - Colberg, J.

AU - Caro, J. F.

PY - 1994

Y1 - 1994

N2 - The objectives of this study were to develop a new technique to safely, reliably, and in a cosmetically acceptable way, obtain more than 5.0 g of abdominal subcutaneous fat in out-patients, and to investigate whether inhibitory effects of a local anaesthetic on adipose tissue function in vitro are sufficient argument against the use of infiltrative local anaesthesia during fat biopsy to obtain samples for metabolic studies. Measurements were obtained to compare glucose transport and lipolysis response to insulin in adipocytes isolated from subcutaneous abdominal fat obtained: (i) during elective surgery in eight women and four men (BMI 25.8 ± 3.1 kg/m2); and (ii) from five male and three female out-patients (BMI 25.8 ± 3.1 kg/m2) by the described novel technique performed under local anaesthesia with Lidocaine. The effects of acute and 11-day exposure to Lidocaine in vitro on adipocyte lipolysis and glucose transport response to insulin, and the growth potential were determined. In vivo exposure of the tissue samples to local anaesthetic by the novel technique had no apparent effect on isolated adipocyte responses to insulin by stimulation of glucose transport or by inhibitor- or adrenaline-stimulated lipolysis; the results were not different to those for adipocytes isolated from fat obtained during elective abdominal surgeries. Lidocaine added in vitro potently inhibited glucose transport and lipolysis in adipocytes, and cell attachment and growth in primary 'ceiling' culture, this effect persisted only as long as Lidocaine was present. After washing, adipocytes fully regained their function and growth regardless of the exposure period, as short as 30 min or as long as 11 days. Therefore, the proposed novel technique allows rapid sampling of significant amounts of abdominal fat in outpatients. Lidocaine has an inhibitory effect on adipocyte function; however, this effect appears to be fully reversible.

AB - The objectives of this study were to develop a new technique to safely, reliably, and in a cosmetically acceptable way, obtain more than 5.0 g of abdominal subcutaneous fat in out-patients, and to investigate whether inhibitory effects of a local anaesthetic on adipose tissue function in vitro are sufficient argument against the use of infiltrative local anaesthesia during fat biopsy to obtain samples for metabolic studies. Measurements were obtained to compare glucose transport and lipolysis response to insulin in adipocytes isolated from subcutaneous abdominal fat obtained: (i) during elective surgery in eight women and four men (BMI 25.8 ± 3.1 kg/m2); and (ii) from five male and three female out-patients (BMI 25.8 ± 3.1 kg/m2) by the described novel technique performed under local anaesthesia with Lidocaine. The effects of acute and 11-day exposure to Lidocaine in vitro on adipocyte lipolysis and glucose transport response to insulin, and the growth potential were determined. In vivo exposure of the tissue samples to local anaesthetic by the novel technique had no apparent effect on isolated adipocyte responses to insulin by stimulation of glucose transport or by inhibitor- or adrenaline-stimulated lipolysis; the results were not different to those for adipocytes isolated from fat obtained during elective abdominal surgeries. Lidocaine added in vitro potently inhibited glucose transport and lipolysis in adipocytes, and cell attachment and growth in primary 'ceiling' culture, this effect persisted only as long as Lidocaine was present. After washing, adipocytes fully regained their function and growth regardless of the exposure period, as short as 30 min or as long as 11 days. Therefore, the proposed novel technique allows rapid sampling of significant amounts of abdominal fat in outpatients. Lidocaine has an inhibitory effect on adipocyte function; however, this effect appears to be fully reversible.

KW - Adipose tissue biopsy

KW - Glucose transport

KW - Lidocaine

KW - Lipolysis

UR - http://www.scopus.com/inward/record.url?scp=0028220457&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028220457&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 161

EP - 166

JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

IS - 3

ER -