A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas

Stephen R. Hann, Michael King, David L. Bentley, Carl W. Anderson, Robert N. Eisenman

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

The c-myc gene comprises three expns with a single large AUG-initiated open reading frame extending from exon 2 through exon 3. Exon 1 lacks any AUG codons. Cells from a wide range of species produce two c-myc proteins that, while highly related, do not appear to arise from posttranslational interconversion. To understand the origin of the two proteins, we mapped them and analyzed the in vitro protein-coding capacity of c-myc cDNAs. Our findings show that the two proteins are derived from alternative translational initiations at the exon 2 AUG and at a non-AUG codon near the 3′ end of exon 1, resulting in the production of proteins with distinct N termini. In Burkitt's lymphomas, the removal or specific mutation of exon 1 in c-myc translocations correlates with suppression of synthesis of the larger protein, and thus may contribute to the oncogenic activation of c-myc.

Original languageEnglish (US)
Pages (from-to)185-195
Number of pages11
JournalCell
Volume52
Issue number2
DOIs
StatePublished - Jan 29 1988
Externally publishedYes

Fingerprint

Burkitt Lymphoma
Exons
Proteins
Codon
Proto-Oncogene Proteins c-myc
myc Genes
Open Reading Frames
Complementary DNA
Genes
Chemical activation
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas. / Hann, Stephen R.; King, Michael; Bentley, David L.; Anderson, Carl W.; Eisenman, Robert N.

In: Cell, Vol. 52, No. 2, 29.01.1988, p. 185-195.

Research output: Contribution to journalArticle

Hann, Stephen R. ; King, Michael ; Bentley, David L. ; Anderson, Carl W. ; Eisenman, Robert N. / A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas. In: Cell. 1988 ; Vol. 52, No. 2. pp. 185-195.
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