A noncytotoxic DsRed variant for whole-cell labeling

Rita L. Strack, Daniel E. Strongin, Dibyendu Bhattacharyya, Wen Tao, Allison Berman, Hal E. Broxmeyer, Robert J. Keenan, Benjamin S. Glick

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

Original languageEnglish (US)
Pages (from-to)955-957
Number of pages3
JournalNature Methods
Volume5
Issue number11
DOIs
StatePublished - Oct 27 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'A noncytotoxic DsRed variant for whole-cell labeling'. Together they form a unique fingerprint.

  • Cite this

    Strack, R. L., Strongin, D. E., Bhattacharyya, D., Tao, W., Berman, A., Broxmeyer, H. E., Keenan, R. J., & Glick, B. S. (2008). A noncytotoxic DsRed variant for whole-cell labeling. Nature Methods, 5(11), 955-957. https://doi.org/10.1038/nmeth.1264