A noncytotoxic DsRed variant for whole-cell labeling

Rita L. Strack, Daniel E. Strongin, Dibyendu Bhattacharyya, Wen Tao, Allison Berman, Hal Broxmeyer, Robert J. Keenan, Benjamin S. Glick

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

Original languageEnglish
Pages (from-to)955-957
Number of pages3
JournalNature Methods
Volume5
Issue number11
DOIs
StatePublished - 2008

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Labeling
Phototoxic Dermatitis
Cytotoxicity
Labels
Proteins
Cells
fluorescent protein 583

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Strack, R. L., Strongin, D. E., Bhattacharyya, D., Tao, W., Berman, A., Broxmeyer, H., ... Glick, B. S. (2008). A noncytotoxic DsRed variant for whole-cell labeling. Nature Methods, 5(11), 955-957. https://doi.org/10.1038/nmeth.1264

A noncytotoxic DsRed variant for whole-cell labeling. / Strack, Rita L.; Strongin, Daniel E.; Bhattacharyya, Dibyendu; Tao, Wen; Berman, Allison; Broxmeyer, Hal; Keenan, Robert J.; Glick, Benjamin S.

In: Nature Methods, Vol. 5, No. 11, 2008, p. 955-957.

Research output: Contribution to journalArticle

Strack, RL, Strongin, DE, Bhattacharyya, D, Tao, W, Berman, A, Broxmeyer, H, Keenan, RJ & Glick, BS 2008, 'A noncytotoxic DsRed variant for whole-cell labeling', Nature Methods, vol. 5, no. 11, pp. 955-957. https://doi.org/10.1038/nmeth.1264
Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer H et al. A noncytotoxic DsRed variant for whole-cell labeling. Nature Methods. 2008;5(11):955-957. https://doi.org/10.1038/nmeth.1264
Strack, Rita L. ; Strongin, Daniel E. ; Bhattacharyya, Dibyendu ; Tao, Wen ; Berman, Allison ; Broxmeyer, Hal ; Keenan, Robert J. ; Glick, Benjamin S. / A noncytotoxic DsRed variant for whole-cell labeling. In: Nature Methods. 2008 ; Vol. 5, No. 11. pp. 955-957.
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