A noncytotoxic DsRed variant for whole-cell labeling

Rita L. Strack, Robert J. Keenan, Daniel E. Strongin, Dibyendu Bhattachryya, Allison Berman, Benjamin S. Glick, Tao Wen, Hal Broxmeyer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fluorescent proteins (FPs) are extremely useful tools for whole-cell, tissue, and animal labeling. For these purposes, FPs may be monomeric or oligomeric, but should meet the criteria of being tolerated at high expression levels in cells and having desirable photophysical properties. Our goal was to create a variant of DsRed-Express that maintains the brightness,fast-maturation, and photostability of this protein, while exhibiting decreased cytotoxicity. For this purpose, we mutated the surface of DsRed-Express to decrease aggregation and created DsRed-Express2. DsRed-Express2 retains the favorable photophysical properties of DsRed-Express while showing dramatically reduced cytotoxicity and higher expression in bacterial and mammalian systems. Further, it was shown that DsRed-Express2 outper forms other red FPs as a label for bacterial and mammalian cells.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7191
DOIs
StatePublished - 2009
EventFluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV - San Jose, CA, United States
Duration: Jan 25 2009Jan 26 2009

Other

OtherFluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV
CountryUnited States
CitySan Jose, CA
Period1/25/091/26/09

Fingerprint

Labeling
marking
proteins
Proteins
Cytotoxicity
cells
animals
Labels
Luminance
brightness
Animals
Agglomeration
Cells
Tissue
fluorescent protein 583

Keywords

  • Directed evolution
  • DsRed
  • Fluorescent protein
  • Whole-cell labeling

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Strack, R. L., Keenan, R. J., Strongin, D. E., Bhattachryya, D., Berman, A., Glick, B. S., ... Broxmeyer, H. (2009). A noncytotoxic DsRed variant for whole-cell labeling. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7191). [719109] https://doi.org/10.1117/12.808046

A noncytotoxic DsRed variant for whole-cell labeling. / Strack, Rita L.; Keenan, Robert J.; Strongin, Daniel E.; Bhattachryya, Dibyendu; Berman, Allison; Glick, Benjamin S.; Wen, Tao; Broxmeyer, Hal.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7191 2009. 719109.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Strack, RL, Keenan, RJ, Strongin, DE, Bhattachryya, D, Berman, A, Glick, BS, Wen, T & Broxmeyer, H 2009, A noncytotoxic DsRed variant for whole-cell labeling. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7191, 719109, Fluorescence In Vivo Imaging Based on Genetically Engineered Probes: From Living Cells to Whole Body Imaging IV, San Jose, CA, United States, 1/25/09. https://doi.org/10.1117/12.808046
Strack RL, Keenan RJ, Strongin DE, Bhattachryya D, Berman A, Glick BS et al. A noncytotoxic DsRed variant for whole-cell labeling. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7191. 2009. 719109 https://doi.org/10.1117/12.808046
Strack, Rita L. ; Keenan, Robert J. ; Strongin, Daniel E. ; Bhattachryya, Dibyendu ; Berman, Allison ; Glick, Benjamin S. ; Wen, Tao ; Broxmeyer, Hal. / A noncytotoxic DsRed variant for whole-cell labeling. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7191 2009.
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