A novel antimicrobial peptidoglycan recognition protein in the cornea

Amit Ghosh, Seakwoo Lee, Roman Dziarski, Shukti Chakravarti

Research output: Contribution to journalArticle

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Abstract

PURPOSE. In an earlier gene expression study, the authors identified a novel antimicrobial gene, Peptidoglycan recognition protein 1 (Pglyrp1), in the mouse cornea. Here the expression of the Pglyrp1 transcript and the encoded protein, PGLYRP1, in the cornea was investigated. The role of PGLYRP1 in the cornea was further investigated using wild-type and Pglyrp1- deficient mice. This is the first report of this antimicrobial protein in the cornea. METHODS. PGLYRP1 was detected in the cornea and was further localized to the epithelium by immunohistology, confocal microscopy, immunoblotting, and real-time PCR. The role of PGLYRP1 in the cornea was investigated by comparing the response of wild-type and Pglyrp1-/- mice to corneal epithelial wounds and Pseudomonas aeruginosa-mediated corneal infections. The antibacterial effects of corneal PGLYRP1 were assayed by measuring bacterial growth in vitro, in the presence of wild-type corneal epithelial extracts, before and after antibody-mediated blocking of PGLYRP1. RESULTS. PGLYRP1 is expressed at high levels in the mouse corneal epithelium. PGLYRP1 was localized to the mouse corneal epithelium and the human corneal epithelium. The Pglyrp1-/- mouse shows delayed healing and poor clearing of bacterial keratitis; in vitro its epithelial protein extract shows reduced bacteriostatic activity compared with wild-type mice. CONCLUSIONS. PGLYRP1 is a novel antimicrobial protein of the corneal epithelium and protects the ocular surface from bacterial infections.

Original languageEnglish
Pages (from-to)4185-4191
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume50
Issue number9
DOIs
StatePublished - 2009

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Cornea
Corneal Epithelium
Bacterial Eye Infections
Proteins
Blocking Antibodies
Keratitis
Immunoblotting
Confocal Microscopy
Pseudomonas aeruginosa
peptidoglycan recognition protein
Real-Time Polymerase Chain Reaction
Epithelium
Gene Expression
Mouse Pglyrp1 protein
Wounds and Injuries
Growth
Infection
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

A novel antimicrobial peptidoglycan recognition protein in the cornea. / Ghosh, Amit; Lee, Seakwoo; Dziarski, Roman; Chakravarti, Shukti.

In: Investigative Ophthalmology and Visual Science, Vol. 50, No. 9, 2009, p. 4185-4191.

Research output: Contribution to journalArticle

Ghosh, Amit ; Lee, Seakwoo ; Dziarski, Roman ; Chakravarti, Shukti. / A novel antimicrobial peptidoglycan recognition protein in the cornea. In: Investigative Ophthalmology and Visual Science. 2009 ; Vol. 50, No. 9. pp. 4185-4191.
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