A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

Ghaeth H. Yassen, T.M. Gabriel Chu, Maxime A. Gallant, Matthew Allen, Mychel M. Vail, Peter E. Murray, Jeffrey Platt

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: The aim of this study was to investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration. Materials and methods: Immature human premolars were selected (n = 22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of the three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide (Ca(OH)2)) or neutral deionized water (control) for 1 or 4 weeks. After each time interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's protected least significant differences were used for statistical analyses. Results: Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP-treated dentine had the highest significant indentation parameters, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Furthermore, TAP-treated dentine had the lowest significant estimated hardness, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Conclusion: BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments. Clinical relevance: The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.

Original languageEnglish
Pages (from-to)1569-1575
Number of pages7
JournalClinical Oral Investigations
Volume18
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Dental Pulp Cavity
Endodontics
Dentin
Regeneration
Hardness
Anti-Bacterial Agents
Calcium Hydroxide
Bicuspid
Ointments
Analysis of Variance

Keywords

  • BioDent
  • Calcium hydroxide
  • Double-antibiotic paste
  • Endodontic regeneration
  • Triple-antibiotic paste

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation. / Yassen, Ghaeth H.; Chu, T.M. Gabriel; Gallant, Maxime A.; Allen, Matthew; Vail, Mychel M.; Murray, Peter E.; Platt, Jeffrey.

In: Clinical Oral Investigations, Vol. 18, No. 6, 2014, p. 1569-1575.

Research output: Contribution to journalArticle

@article{ecf927e1049b44748e9808821ed66e54,
title = "A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation",
abstract = "Objectives: The aim of this study was to investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration. Materials and methods: Immature human premolars were selected (n = 22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of the three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide (Ca(OH)2)) or neutral deionized water (control) for 1 or 4 weeks. After each time interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's protected least significant differences were used for statistical analyses. Results: Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP-treated dentine had the highest significant indentation parameters, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Furthermore, TAP-treated dentine had the lowest significant estimated hardness, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Conclusion: BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments. Clinical relevance: The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.",
keywords = "BioDent, Calcium hydroxide, Double-antibiotic paste, Endodontic regeneration, Triple-antibiotic paste",
author = "Yassen, {Ghaeth H.} and Chu, {T.M. Gabriel} and Gallant, {Maxime A.} and Matthew Allen and Vail, {Mychel M.} and Murray, {Peter E.} and Jeffrey Platt",
year = "2014",
doi = "10.1007/s00784-013-1125-x",
language = "English",
volume = "18",
pages = "1569--1575",
journal = "Clinical Oral Investigations",
issn = "1432-6981",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - A novel approach to evaluate the effect of medicaments used in endodontic regeneration on root canal surface indentation

AU - Yassen, Ghaeth H.

AU - Chu, T.M. Gabriel

AU - Gallant, Maxime A.

AU - Allen, Matthew

AU - Vail, Mychel M.

AU - Murray, Peter E.

AU - Platt, Jeffrey

PY - 2014

Y1 - 2014

N2 - Objectives: The aim of this study was to investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration. Materials and methods: Immature human premolars were selected (n = 22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of the three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide (Ca(OH)2)) or neutral deionized water (control) for 1 or 4 weeks. After each time interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's protected least significant differences were used for statistical analyses. Results: Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP-treated dentine had the highest significant indentation parameters, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Furthermore, TAP-treated dentine had the lowest significant estimated hardness, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Conclusion: BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments. Clinical relevance: The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.

AB - Objectives: The aim of this study was to investigate the capability of a novel reference point indentation apparatus to test the indentation properties of root canal surface dentine treated with three intracanal medicaments used in endodontic regeneration. Materials and methods: Immature human premolars were selected (n = 22). Four specimens were obtained from each root and randomly assigned to three treatment groups and a control group. Each specimen was exposed to one of the three treatment pastes (triple antibiotic (TAP), double antibiotic (DAP), or calcium hydroxide (Ca(OH)2)) or neutral deionized water (control) for 1 or 4 weeks. After each time interval, the indentation properties of the root canal dentine surfaces were measured using a BioDent reference point indenter. Two-way ANOVA and Fisher's protected least significant differences were used for statistical analyses. Results: Significant differences in indentation parameters and estimated hardness between all groups at both time points were found. TAP-treated dentine had the highest significant indentation parameters, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Furthermore, TAP-treated dentine had the lowest significant estimated hardness, followed by DAP-treated dentine, untreated control dentine, and Ca(OH)2-treated dentine, respectively. Conclusion: BioDent reference point indenter was able to detect significant differences in indentation properties of root canal dentine treated with various medicaments. Clinical relevance: The use of a reference point indenter is a promising approach to characterize the indentation properties of root canal surfaces without any surface modification. This might provide an in vitro mechanical measurement that is more representative of the actual clinical situation.

KW - BioDent

KW - Calcium hydroxide

KW - Double-antibiotic paste

KW - Endodontic regeneration

KW - Triple-antibiotic paste

UR - http://www.scopus.com/inward/record.url?scp=84902840906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902840906&partnerID=8YFLogxK

U2 - 10.1007/s00784-013-1125-x

DO - 10.1007/s00784-013-1125-x

M3 - Article

VL - 18

SP - 1569

EP - 1575

JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

SN - 1432-6981

IS - 6

ER -