A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors

Byung S. Youn, Ihn K. Jang, Hal Broxmeyer, Scott Cooper, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Teresa A. Elick, Malcolm J. Fraser, Byoung S. Kwon

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

A new member of the β-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1α. MRP- 2 shows a 50.8% sequence identity at the protein level to MRP-1 and a 46.3% identity to MIP-1α. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the β-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst- forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage- megakaryocyte) progenitor cells stimulated by combinations of growth factors.

Original languageEnglish
Pages (from-to)2661-2667
Number of pages7
JournalJournal of Immunology
Volume155
Issue number5
StatePublished - 1995

Fingerprint

Macrophage Inflammatory Proteins
Chemokines
Bone Marrow
Proteins
Macrophages
Granulocytes
Megakaryocyte-Erythroid Progenitor Cells
Amino Acids
Messenger RNA
Erythroid Precursor Cells
Chromosomes, Human, Pair 11
Neurofibromatosis 1
Multigene Family
Protein Sorting Signals
Peroxidase
Monocytes
Intercellular Signaling Peptides and Proteins
B-Lymphocytes
Molecular Weight
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Youn, B. S., Jang, I. K., Broxmeyer, H., Cooper, S., Jenkins, N. A., Gilbert, D. J., ... Kwon, B. S. (1995). A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors. Journal of Immunology, 155(5), 2661-2667.

A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors. / Youn, Byung S.; Jang, Ihn K.; Broxmeyer, Hal; Cooper, Scott; Jenkins, Nancy A.; Gilbert, Debra J.; Copeland, Neal G.; Elick, Teresa A.; Fraser, Malcolm J.; Kwon, Byoung S.

In: Journal of Immunology, Vol. 155, No. 5, 1995, p. 2661-2667.

Research output: Contribution to journalArticle

Youn, BS, Jang, IK, Broxmeyer, H, Cooper, S, Jenkins, NA, Gilbert, DJ, Copeland, NG, Elick, TA, Fraser, MJ & Kwon, BS 1995, 'A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors', Journal of Immunology, vol. 155, no. 5, pp. 2661-2667.
Youn, Byung S. ; Jang, Ihn K. ; Broxmeyer, Hal ; Cooper, Scott ; Jenkins, Nancy A. ; Gilbert, Debra J. ; Copeland, Neal G. ; Elick, Teresa A. ; Fraser, Malcolm J. ; Kwon, Byoung S. / A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors. In: Journal of Immunology. 1995 ; Vol. 155, No. 5. pp. 2661-2667.
@article{caf10f95ac9e4cd59b97c5bebeca8d45,
title = "A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors",
abstract = "A new member of the β-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1α. MRP- 2 shows a 50.8{\%} sequence identity at the protein level to MRP-1 and a 46.3{\%} identity to MIP-1α. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the β-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst- forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage- megakaryocyte) progenitor cells stimulated by combinations of growth factors.",
author = "Youn, {Byung S.} and Jang, {Ihn K.} and Hal Broxmeyer and Scott Cooper and Jenkins, {Nancy A.} and Gilbert, {Debra J.} and Copeland, {Neal G.} and Elick, {Teresa A.} and Fraser, {Malcolm J.} and Kwon, {Byoung S.}",
year = "1995",
language = "English",
volume = "155",
pages = "2661--2667",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors

AU - Youn, Byung S.

AU - Jang, Ihn K.

AU - Broxmeyer, Hal

AU - Cooper, Scott

AU - Jenkins, Nancy A.

AU - Gilbert, Debra J.

AU - Copeland, Neal G.

AU - Elick, Teresa A.

AU - Fraser, Malcolm J.

AU - Kwon, Byoung S.

PY - 1995

Y1 - 1995

N2 - A new member of the β-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1α. MRP- 2 shows a 50.8% sequence identity at the protein level to MRP-1 and a 46.3% identity to MIP-1α. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the β-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst- forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage- megakaryocyte) progenitor cells stimulated by combinations of growth factors.

AB - A new member of the β-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1α. MRP- 2 shows a 50.8% sequence identity at the protein level to MRP-1 and a 46.3% identity to MIP-1α. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the β-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst- forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage- megakaryocyte) progenitor cells stimulated by combinations of growth factors.

UR - http://www.scopus.com/inward/record.url?scp=0029077317&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029077317&partnerID=8YFLogxK

M3 - Article

C2 - 7650394

AN - SCOPUS:0029077317

VL - 155

SP - 2661

EP - 2667

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -