A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors

Byung S. Youn, Ihn K. Jang, Hal E. Broxmeyer, Scott Cooper, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Teresa A. Elick, Malcolm J. Fraser, Byoung S. Kwon

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56 Scopus citations

Abstract

A new member of the β-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1α. MRP- 2 shows a 50.8% sequence identity at the protein level to MRP-1 and a 46.3% identity to MIP-1α. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the β-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst- forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage- megakaryocyte) progenitor cells stimulated by combinations of growth factors.

Original languageEnglish (US)
Pages (from-to)2661-2667
Number of pages7
JournalJournal of Immunology
Volume155
Issue number5
StatePublished - Sep 14 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Youn, B. S., Jang, I. K., Broxmeyer, H. E., Cooper, S., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Elick, T. A., Fraser, M. J., & Kwon, B. S. (1995). A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors. Journal of Immunology, 155(5), 2661-2667.