We have previously described that several lysophospholipids (lysophosphatidic acid, lysophosphatidylserine and sphingosylphosphorylcholine) and other small molecular weight molecules stimulate signaling pathways in ovarian and breast cancer cells (Xu et al. Biochem. J. 309. 933-940, 1995; Clinic. Cancer Res. 1, 1223-1232, 1995). In an effort to isolate the receptor(s) for these small molecules, we have cloned a novel G protein coupled receptor, termed ovarian cancer G protein coupled receptor 1 (OGR1) from ovarian cancer cells by degenerate oligonucleotide polymerase chain reaction (PCR) amplification. We have mapped the OGR1 gene to chromosome 14q31 by FISH analysis. The open reading frame of OGR1 encodes a protein of 365 amino acids. OGR1 is expressed as a single transcript (approximately 3 kb) in several tissues, including spleen, testis, small intestine, peripheral blood leukocytes, brain, heart, lung, placenta and kidney. There is no detectable OGR1 expression in normal ovary, breast, thymus, prostate, colon, liver, skeletal muscle or pancreas. However, northern blot and reverse transcriptase-PCR (RTPCR) analyses show that OGR1 is expressed in the majority of ovarian and breast cancer cell lines and tumors. OGR1 may therefore represent an important regulator in ovarian and breast tumor development.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology