A novel GCN5b lysine acetyltransferase complex associates with distinct transcription factors in the protozoan parasite Toxoplasma gondii

Michael T. Harris, Victoria Jeffers, Jennifer Martynowicz, Jason D. True, Amber Mosley, William Sullivan

Research output: Contribution to journalArticle

Abstract

Toxoplasma gondii is a protozoan parasite that has a tremendous impact on human health and livestock. High seroprevalence among humans and other animals is facilitated by the conversion of rapidly proliferating tachyzoites into latent bradyzoites that are housed in tissue cysts, which allow transmission through predation. Epigenetic mechanisms contribute to the regulation of gene expression events that are crucial in both tachyzoites as well as their development into bradyzoites. Acetylation of histones is one of the critical histone modifications that is linked to active gene transcription. Unlike most early-branching eukaryotes, Toxoplasma possesses two GCN5 homologues, one of which, GCN5b, is essential for parasite viability. Surprisingly, GCN5b does not associate with most of the well-conserved proteins found in the GCN5 complexes of other eukaryotes. Of particular note is that GCN5b interacts with multiple putative transcription factors that have plant-like DNA-binding domains denoted as AP2. To understand the function of GCN5b and its role(s) in epigenetic gene regulation of stage switching, we performed co-immunoprecipitation of GCN5b under normal and bradyzoite induction conditions. We report the greatest resolution of the GCN5b complex to date under these various culture conditions. Moreover, reciprocal co-IPs were performed with distinct GCN5b-interacting AP2 factors (AP2IX-7 and AP2XII-4) to delineate the interactomes of each putative transcription factor. Our findings suggest that GCN5b is associated with at least two distinct complexes that are characterized by two different pairs of AP2 factors, and implicate up to four AP2 proteins to be involved with GCN5b-mediated gene regulation.

Original languageEnglish (US)
Article number111203
JournalMolecular and Biochemical Parasitology
Volume232
DOIs
StatePublished - Sep 1 2019

Fingerprint

Toxoplasma
Acetylation
Epigenomics
Proteomics
Parasites
Transcription Factors
Eukaryota
Histone Code
Plant DNA
Genes
Seroepidemiologic Studies
Gene Expression Regulation
Livestock
Immunoprecipitation
Histones
Cysts
Proteins
Health
Lysine Acetyltransferases

Keywords

  • Acetylation
  • Apicomplexan
  • Epigenetics
  • Proteomics
  • Toxoplasma

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

Cite this

A novel GCN5b lysine acetyltransferase complex associates with distinct transcription factors in the protozoan parasite Toxoplasma gondii. / Harris, Michael T.; Jeffers, Victoria; Martynowicz, Jennifer; True, Jason D.; Mosley, Amber; Sullivan, William.

In: Molecular and Biochemical Parasitology, Vol. 232, 111203, 01.09.2019.

Research output: Contribution to journalArticle

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