A novel in-vitro system for the simultaneous exposure of bladder smooth muscle cells to mechanical strain and sustained hydrostatic pressure

Karen M. Haberstroh, Martin Kaefer, Natacha DePaola, Sarah A. Frommer, Rena Bizios

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The novel hydrostrain system was designed in an effort to establish and maintain conditions that simulate the in-vivo mechanical environment of the bladder. In this laboratory system, ovine bladder smooth muscle cells on flexible, 10-cm-dia silastic membranes were exposed simultaneously to hydrostatic pressure (40 cm H20, a pressure level currently associated with bladder pathologies) and mechanical strains (up to 25 percent) under standard cell culture conditions for 7 h. Under these conditions, Heparin Binding-Epidermal Growth Factor and Collagen Type III mRNA expression were significantly increased (p

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalJournal of Biomechanical Engineering
Volume124
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Hydrostatic Pressure
Pathology
Hydrostatic pressure
Cell culture
Collagen
Smooth Muscle Myocytes
Muscle
Urinary Bladder
Cells
Membranes
Collagen Type III
Epidermal Growth Factor
Heparin
Sheep
Cell Culture Techniques
Pressure
Messenger RNA
In Vitro Techniques

Keywords

  • Bladder
  • Collagen Type I
  • Collagen Type III
  • Heparin Binding-Epidermal Growth Factor
  • Hydrostrain System
  • Mechanical Forces
  • mRNA
  • Smooth Muscle Cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biophysics

Cite this

A novel in-vitro system for the simultaneous exposure of bladder smooth muscle cells to mechanical strain and sustained hydrostatic pressure. / Haberstroh, Karen M.; Kaefer, Martin; DePaola, Natacha; Frommer, Sarah A.; Bizios, Rena.

In: Journal of Biomechanical Engineering, Vol. 124, No. 2, 2002, p. 208-213.

Research output: Contribution to journalArticle

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