A novel in-vitro system for the simultaneous exposure of bladder smooth muscle cells to mechanical strain and sustained hydrostatic pressure

Karen M. Haberstroh, Martin Kaefer, Natacha DePaola, Sarah A. Frommer, Rena Bizios

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The novel hydrostrain system was designed in an effort to establish and maintain conditions that simulate the in-vivo mechanical environment of the bladder. In this laboratory system, ovine bladder smooth muscle cells on flexible, 10-cm-dia silastic membranes were exposed simultaneously to hydrostatic pressure (40 cm H20, a pressure level currently associated with bladder pathologies) and mechanical strains (up to 25 percent) under standard cell culture conditions for 7 h. Under these conditions, Heparin Binding-Epidermal Growth Factor and Collagen Type III mRNA expression were significantly increased (p<0.01 and 0.1, respectively); however, no changes were observed in Collagen Type I mRNA expression. Decreases in the Collagen Type I: Type III ratio following simultaneous exposure of bladder smooth muscle cells to pathological levels of hydrostatic pressure and mechanical strain in vitro are in agreement with clinically observed increases in Collagen Type III with concomitant decreased human bladder compliance. The results of the present study, therefore, provide cellular/molecular level information relevant to bladder pathology that could have significant implications in the field of clinical urology.

Original languageEnglish (US)
Pages (from-to)208-213
Number of pages6
JournalJournal of Biomechanical Engineering
Volume124
Issue number2
DOIs
StatePublished - Apr 29 2002
Externally publishedYes

Keywords

  • Bladder
  • Collagen Type I
  • Collagen Type III
  • Heparin Binding-Epidermal Growth Factor
  • Hydrostrain System
  • Mechanical Forces
  • Smooth Muscle Cells
  • mRNA

ASJC Scopus subject areas

  • Biomedical Engineering
  • Physiology (medical)

Fingerprint Dive into the research topics of 'A novel in-vitro system for the simultaneous exposure of bladder smooth muscle cells to mechanical strain and sustained hydrostatic pressure'. Together they form a unique fingerprint.

  • Cite this