A novel method of myeloablation to enhance engraftment of adult bone marrow cells in newborn mice

Mervin Yoder, James G. Cumming, Kelly Hiatt, Pinku Mukherjee, David A. Williams

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

This report describes the successful development of a transplantation model in which we engrafted adult murine bone marrow hematopoietic cells in congenic day-old murine pups. Newborn animals conditioned with high doses (≥7.0 Gy) of total-body irradiation (TBI) demonstrated severe growth retardation and untoward effects on the developing central nervous system. Newborn pups conditioned with a sublethal dose (5.0 Gy) of TBI, however, achieved normal growth and development up to 6 months posttransplantation, and donor type hematopoietic cell reconstitution exceeded 50% in these animals. A second conditioning regimen, comprising one or two doses of busulfan (15 mg/kg) administered intrapartum, resulted in significant myelosuppression in untransplanted new-born pups. This second regimen was associated, however, with engraftment and full multilineage reconstitution of the conditioned newborn recipient animals with adult bone marrow cells, and the engrafted pups grew and developed without apparent defect. A third conditioning regimen combining a single dose of busulfan with sublethal TBI also permitted engraftment and full multilineage reconstitution of the recipient animals. Thus, successful transplantation of hematopoietic cells into newborn recipient mice may be achieved without significant morbidity if a specific conditioning regimen is employed.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalBiology of Blood and Marrow Transplantation
Volume2
Issue number2
StatePublished - May 1996

Fingerprint

Whole-Body Irradiation
Bone Marrow Cells
Newborn Animals
Busulfan
Cell Transplantation
Growth and Development
Central Nervous System
Transplantation
Morbidity
Growth

Keywords

  • Busulfan
  • Newborn mice
  • Transplantation

ASJC Scopus subject areas

  • Transplantation

Cite this

A novel method of myeloablation to enhance engraftment of adult bone marrow cells in newborn mice. / Yoder, Mervin; Cumming, James G.; Hiatt, Kelly; Mukherjee, Pinku; Williams, David A.

In: Biology of Blood and Marrow Transplantation, Vol. 2, No. 2, 05.1996, p. 59-67.

Research output: Contribution to journalArticle

Yoder, Mervin ; Cumming, James G. ; Hiatt, Kelly ; Mukherjee, Pinku ; Williams, David A. / A novel method of myeloablation to enhance engraftment of adult bone marrow cells in newborn mice. In: Biology of Blood and Marrow Transplantation. 1996 ; Vol. 2, No. 2. pp. 59-67.
@article{e93e84d24d75453a8d50390440eb0cfb,
title = "A novel method of myeloablation to enhance engraftment of adult bone marrow cells in newborn mice",
abstract = "This report describes the successful development of a transplantation model in which we engrafted adult murine bone marrow hematopoietic cells in congenic day-old murine pups. Newborn animals conditioned with high doses (≥7.0 Gy) of total-body irradiation (TBI) demonstrated severe growth retardation and untoward effects on the developing central nervous system. Newborn pups conditioned with a sublethal dose (5.0 Gy) of TBI, however, achieved normal growth and development up to 6 months posttransplantation, and donor type hematopoietic cell reconstitution exceeded 50{\%} in these animals. A second conditioning regimen, comprising one or two doses of busulfan (15 mg/kg) administered intrapartum, resulted in significant myelosuppression in untransplanted new-born pups. This second regimen was associated, however, with engraftment and full multilineage reconstitution of the conditioned newborn recipient animals with adult bone marrow cells, and the engrafted pups grew and developed without apparent defect. A third conditioning regimen combining a single dose of busulfan with sublethal TBI also permitted engraftment and full multilineage reconstitution of the recipient animals. Thus, successful transplantation of hematopoietic cells into newborn recipient mice may be achieved without significant morbidity if a specific conditioning regimen is employed.",
keywords = "Busulfan, Newborn mice, Transplantation",
author = "Mervin Yoder and Cumming, {James G.} and Kelly Hiatt and Pinku Mukherjee and Williams, {David A.}",
year = "1996",
month = "5",
language = "English",
volume = "2",
pages = "59--67",
journal = "Biology of Blood and Marrow Transplantation",
issn = "1083-8791",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - A novel method of myeloablation to enhance engraftment of adult bone marrow cells in newborn mice

AU - Yoder, Mervin

AU - Cumming, James G.

AU - Hiatt, Kelly

AU - Mukherjee, Pinku

AU - Williams, David A.

PY - 1996/5

Y1 - 1996/5

N2 - This report describes the successful development of a transplantation model in which we engrafted adult murine bone marrow hematopoietic cells in congenic day-old murine pups. Newborn animals conditioned with high doses (≥7.0 Gy) of total-body irradiation (TBI) demonstrated severe growth retardation and untoward effects on the developing central nervous system. Newborn pups conditioned with a sublethal dose (5.0 Gy) of TBI, however, achieved normal growth and development up to 6 months posttransplantation, and donor type hematopoietic cell reconstitution exceeded 50% in these animals. A second conditioning regimen, comprising one or two doses of busulfan (15 mg/kg) administered intrapartum, resulted in significant myelosuppression in untransplanted new-born pups. This second regimen was associated, however, with engraftment and full multilineage reconstitution of the conditioned newborn recipient animals with adult bone marrow cells, and the engrafted pups grew and developed without apparent defect. A third conditioning regimen combining a single dose of busulfan with sublethal TBI also permitted engraftment and full multilineage reconstitution of the recipient animals. Thus, successful transplantation of hematopoietic cells into newborn recipient mice may be achieved without significant morbidity if a specific conditioning regimen is employed.

AB - This report describes the successful development of a transplantation model in which we engrafted adult murine bone marrow hematopoietic cells in congenic day-old murine pups. Newborn animals conditioned with high doses (≥7.0 Gy) of total-body irradiation (TBI) demonstrated severe growth retardation and untoward effects on the developing central nervous system. Newborn pups conditioned with a sublethal dose (5.0 Gy) of TBI, however, achieved normal growth and development up to 6 months posttransplantation, and donor type hematopoietic cell reconstitution exceeded 50% in these animals. A second conditioning regimen, comprising one or two doses of busulfan (15 mg/kg) administered intrapartum, resulted in significant myelosuppression in untransplanted new-born pups. This second regimen was associated, however, with engraftment and full multilineage reconstitution of the conditioned newborn recipient animals with adult bone marrow cells, and the engrafted pups grew and developed without apparent defect. A third conditioning regimen combining a single dose of busulfan with sublethal TBI also permitted engraftment and full multilineage reconstitution of the recipient animals. Thus, successful transplantation of hematopoietic cells into newborn recipient mice may be achieved without significant morbidity if a specific conditioning regimen is employed.

KW - Busulfan

KW - Newborn mice

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=0030133629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030133629&partnerID=8YFLogxK

M3 - Article

C2 - 9118300

AN - SCOPUS:0030133629

VL - 2

SP - 59

EP - 67

JO - Biology of Blood and Marrow Transplantation

JF - Biology of Blood and Marrow Transplantation

SN - 1083-8791

IS - 2

ER -