A novel spatially designed and functionally graded electrospun membrane for periodontal regeneration

Marco C. Bottino, Vinoy Thomas, Gregg M. Janowski

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

A periodontal membrane with a graded structure allows tailoring of the layer properties to design a material system that will retain its physical, chemical and mechanical characteristics for a period long enough to optimize periodontal regeneration. In this work a novel functionally graded membrane (FGM) was designed and fabricated via sequential multilayer electrospinning. The FGM consists of a core layer (CL) and two functional surface layers (SLs) interfacing with bone (nano-hydroxyapatite, n-HAp) and epithelial (metronidazole, MET) tissues. The CL comprises a neat poly(dl-lactide-co - caprolactone) (PLCL) layer surrounded by two composite layers composed of a protein/polymer ternary blend (PLCL:PLA:GEL). Electrospinning parameters involved in fabrication of the individual layers (i.e. neat PLCL, ternary blend, PLA:GEL + 10%n-HAp and PLA:GEL + 25%MET) were optimized to obtain fibrous layers free of beads. Morphology, structure and mechanical property studies were carried out on each electrospun layer. The individual fiber morphology and roughness of the functional SLs, which are the n-HAp containing and drug-incorporating layers were evaluated by atomic force microscopy. The CL structure demonstrated higher strength (8.7 MPa) and a more elastic behavior (strain at break 357%) compared with the FGM (3.5 MPa, 297%). Incorporation of n-HAp to enhance osteoconductive behavior and MET to combat periodontal pathogens led to a novel FGM that holds promise at solving the drawbacks of currently available membranes.

Original languageEnglish (US)
Pages (from-to)216-224
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Regeneration
Durapatite
Membranes
Hydroxyapatite
Metronidazole
Electrospinning
Atomic Force Microscopy
Pathogens
Atomic force microscopy
Polymers
Bone
Multilayers
Epithelium
Surface roughness
Tissue
Proteins
Bone and Bones
Fabrication
Mechanical properties
Fibers

Keywords

  • Electrospinning
  • Graded structure
  • Membranes
  • Periodontal regeneration
  • Periodontitis

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

A novel spatially designed and functionally graded electrospun membrane for periodontal regeneration. / Bottino, Marco C.; Thomas, Vinoy; Janowski, Gregg M.

In: Acta Biomaterialia, Vol. 7, No. 1, 01.01.2011, p. 216-224.

Research output: Contribution to journalArticle

Bottino, Marco C. ; Thomas, Vinoy ; Janowski, Gregg M. / A novel spatially designed and functionally graded electrospun membrane for periodontal regeneration. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 1. pp. 216-224.
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