A one-step method for detecting and typing human papillomavirus dna in cervical scrape specimens from women with cervical dysplasia

Douglas H. Webb, Robert E. Rogers, Kenneth Fife

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We studied 66 women with a previous dysplastic cervical cytological smear who were referred for colposcopy and biopsy for the presence of human papillomavirus (HPV) types 6, 11, 16, 18, and 31 DNA in cervical specimens. The specimens were analyzed by a novel hybridization method termed reverse blotting, in which cellular DNA is radiolabeled and used to probe a battery of cloned HPV DNAs immobilized on nitrocellulose. Reconstruction experiments demonstrated that this method could detect about one HPV genome equivalent per cell. HPV DNA sequences were detected in 52 (96%) of 54 patients who showed either condylomatous changes or dysplasia by cervical biopsy. HPV-16 was most commonly detected overall and was detected in 61% of moderate or severe dysplastic samples. HPV DNA was also detected in seven of 12 cervical scrapes from women with a history of dysplasia but with either normal or inflammatory changes noted on cervical biopsy. Our results indicate that the reverse-blot method can detect DNA homologous to various HPV types in a single experiment using DNA from the small numbers of cells obtained by cervical scraping.

Original languageEnglish (US)
Pages (from-to)912-919
Number of pages8
JournalJournal of Infectious Diseases
Volume156
Issue number6
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Uterine Cervical Dysplasia
DNA
Biopsy
Human papillomavirus 31
Immobilized Nucleic Acids
Human papillomavirus 11
Human papillomavirus 6
Colposcopy
Vaginal Smears
Collodion
Human papillomavirus 16
Human Genome
Cell Count

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

A one-step method for detecting and typing human papillomavirus dna in cervical scrape specimens from women with cervical dysplasia. / Webb, Douglas H.; Rogers, Robert E.; Fife, Kenneth.

In: Journal of Infectious Diseases, Vol. 156, No. 6, 1987, p. 912-919.

Research output: Contribution to journalArticle

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