A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis

Zeinab Ebrahimzadeh, Angana Mukherjee, Marie Ève Crochetière, Audrey Sergerie, Souad Amiar, L. Alexa Thompson, Dominic Gagnon, David Gaumond, Robert Stahelin, Joel B. Dacks, Dave Richard

Research output: Contribution to journalArticle

Abstract

Invasion of human red blood cells by the malaria parasite Plasmodium falciparum is an essential step in the development of the disease. Consequently, the molecular players involved in host cell invasion represent important targets for inhibitor design and vaccine development. The process of merozoite invasion is a succession of steps underlined by the sequential secretion of the organelles of the apical complex. However, little is known with regard to how their contents are exocytosed. Here, we identify a phosphoinositide-binding protein conserved in apicomplexan parasites and show that it is important for the attachment and subsequent invasion of the erythrocyte by the merozoite. Critically, removing the protein from its site of action by knock sideways preferentially prevents the secretion of certain types of micronemes. Our results therefore provide evidence for a role of phosphoinositide lipids in the malaria invasion process and provide further insight into the secretion of microneme organelle populations, which is potentially applicable to diverse apicomplexan parasites.

Original languageEnglish (US)
Article numbere47102
JournalEMBO Reports
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Exocytosis
Phosphatidylinositols
Merozoites
Carrier Proteins
Parasites
Organelles
Erythrocytes
Falciparum Malaria
Malaria
Blood
Vaccines
Cells
Lipids
Population
Proteins

Keywords

  • exocytosis
  • invasion
  • malaria
  • microneme

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Ebrahimzadeh, Z., Mukherjee, A., Crochetière, M. È., Sergerie, A., Amiar, S., Thompson, L. A., ... Richard, D. (2019). A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. EMBO Reports, [e47102]. https://doi.org/10.15252/embr.201847102

A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. / Ebrahimzadeh, Zeinab; Mukherjee, Angana; Crochetière, Marie Ève; Sergerie, Audrey; Amiar, Souad; Thompson, L. Alexa; Gagnon, Dominic; Gaumond, David; Stahelin, Robert; Dacks, Joel B.; Richard, Dave.

In: EMBO Reports, 01.01.2019.

Research output: Contribution to journalArticle

Ebrahimzadeh, Z, Mukherjee, A, Crochetière, MÈ, Sergerie, A, Amiar, S, Thompson, LA, Gagnon, D, Gaumond, D, Stahelin, R, Dacks, JB & Richard, D 2019, 'A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis', EMBO Reports. https://doi.org/10.15252/embr.201847102
Ebrahimzadeh Z, Mukherjee A, Crochetière MÈ, Sergerie A, Amiar S, Thompson LA et al. A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. EMBO Reports. 2019 Jan 1. e47102. https://doi.org/10.15252/embr.201847102
Ebrahimzadeh, Zeinab ; Mukherjee, Angana ; Crochetière, Marie Ève ; Sergerie, Audrey ; Amiar, Souad ; Thompson, L. Alexa ; Gagnon, Dominic ; Gaumond, David ; Stahelin, Robert ; Dacks, Joel B. ; Richard, Dave. / A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis. In: EMBO Reports. 2019.
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