A permease-oxidase complex involved in high-affinity iron uptake in yeast

Robert Stearman, Daniel S. Yuan, Yuko Yamaguchi-Iwai, Richard D. Klausner, Andrew Dancis

Research output: Contribution to journalArticle

528 Citations (Scopus)

Abstract

Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast - a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene - were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTRI expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.

Original languageEnglish (US)
Pages (from-to)1552-1557
Number of pages6
JournalScience
Volume271
Issue number5255
StatePublished - Mar 15 1996
Externally publishedYes

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Membrane Transport Proteins
Oxidoreductases
Iron
Yeasts
Proteins
Cell Membrane
Conserved Sequence
Genes
Copper
Mutation
Membranes
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Stearman, R., Yuan, D. S., Yamaguchi-Iwai, Y., Klausner, R. D., & Dancis, A. (1996). A permease-oxidase complex involved in high-affinity iron uptake in yeast. Science, 271(5255), 1552-1557.

A permease-oxidase complex involved in high-affinity iron uptake in yeast. / Stearman, Robert; Yuan, Daniel S.; Yamaguchi-Iwai, Yuko; Klausner, Richard D.; Dancis, Andrew.

In: Science, Vol. 271, No. 5255, 15.03.1996, p. 1552-1557.

Research output: Contribution to journalArticle

Stearman, R, Yuan, DS, Yamaguchi-Iwai, Y, Klausner, RD & Dancis, A 1996, 'A permease-oxidase complex involved in high-affinity iron uptake in yeast', Science, vol. 271, no. 5255, pp. 1552-1557.
Stearman R, Yuan DS, Yamaguchi-Iwai Y, Klausner RD, Dancis A. A permease-oxidase complex involved in high-affinity iron uptake in yeast. Science. 1996 Mar 15;271(5255):1552-1557.
Stearman, Robert ; Yuan, Daniel S. ; Yamaguchi-Iwai, Yuko ; Klausner, Richard D. ; Dancis, Andrew. / A permease-oxidase complex involved in high-affinity iron uptake in yeast. In: Science. 1996 ; Vol. 271, No. 5255. pp. 1552-1557.
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