A permease-oxidase complex involved in high-affinity iron uptake in yeast

Robert Stearman, Daniel S. Yuan, Yuko Yamaguchi-Iwai, Richard D. Klausner, Andrew Dancis

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534 Scopus citations

Abstract

Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast - a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene - were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTRI expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.

Original languageEnglish (US)
Pages (from-to)1552-1557
Number of pages6
JournalScience
Volume271
Issue number5255
DOIs
StatePublished - Mar 15 1996
Externally publishedYes

ASJC Scopus subject areas

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    Stearman, R., Yuan, D. S., Yamaguchi-Iwai, Y., Klausner, R. D., & Dancis, A. (1996). A permease-oxidase complex involved in high-affinity iron uptake in yeast. Science, 271(5255), 1552-1557. https://doi.org/10.1126/science.271.5255.1552