A pharmacogenetics study to predict outcome in patients receiving anti-VEGF therapy in age related macular degeneration

John W. Kitchens; Nawal Kassem; William Wood; Thomas W. Stone; Rick Isernhagen; Edward Wood; Brad A. Hancock; Milan Radovich; Josh Waymire; Lang Li; Bryan P. Schneider

doi:10.2147/OPTH.S39635

A pharmacogenetics study to predict outcome in patients receiving anti-VEGF therapy in age related macular degeneration

John W. Kitchens, Nawal Kassem, William Wood, Thomas W. Stone, Rick Isernhagen, Edward Wood, Brad A. Hancock, Milan Radovich, Josh Waymire, Lang Li, Bryan P. Schneider

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Purpose: To ascertain whether single nucleotide polymorphisms (SNPs) in the Vascular Endothelial Growth factor (VEGFA), Complement Factor H (CFH), and LOC387715 genes could predict outcome to anti-VEGF therapy for patients with age related macular degeneration (AMD). Methods: Patients with "wet" AMD were identified by chart review. Baseline optical coherence tomography (OCT) and visual acuity (VA) data, and at least 6 months of clinical follow up after 3 initial monthly injections of bevacizumab or ranibizumab were required for inclusion. Based on OCT and VA, patients were categorized into two possible clinical outcomes: (a) responders and (b) non-responders. DNA was extracted from saliva and genotyped for candidate SNPs in the VEGFA, LOC387715, and CFH genes. Clinical outcomes were statistically compared to patient genotypes. Results: 101 patients were recruited, and one eye from each patient was included in the analysis. 97% of samples were successfully genotyped for all SNPs. We found a statistically significant association between the LOC387715 A69S TT genotype and outcome based on OCT. Conclusion: Genetic variation may be associated with outcome in patients receiving anti-VEGF therapy.

Original language	English (US)
Pages (from-to)	1987-1993
Number of pages	7
Journal	Clinical Ophthalmology
Volume	7
DOIs	https://doi.org/10.2147/OPTH.S39635
State	Published - Oct 9 2013

Keywords

Age related macular degeneration
ARMS2
Bevacizumab
Complement factor H (CFH)
LOC387715
Ranibizumab
Single nucleotide polymorphisms
Vascular endothelial growth factor

ASJC Scopus subject areas

Ophthalmology

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10.2147/OPTH.S39635

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A pharmacogenetics study to predict outcome in patients receiving anti-VEGF therapy in age related macular degeneration. / Kitchens, John W.; Kassem, Nawal; Wood, William; Stone, Thomas W.; Isernhagen, Rick; Wood, Edward; Hancock, Brad A.; Radovich, Milan; Waymire, Josh; Li, Lang; Schneider, Bryan P.

In: Clinical Ophthalmology, Vol. 7, 09.10.2013, p. 1987-1993.

Research output: Contribution to journal › Article › peer-review

Kitchens, John W. ; Kassem, Nawal ; Wood, William ; Stone, Thomas W. ; Isernhagen, Rick ; Wood, Edward ; Hancock, Brad A. ; Radovich, Milan ; Waymire, Josh ; Li, Lang ; Schneider, Bryan P. / A pharmacogenetics study to predict outcome in patients receiving anti-VEGF therapy in age related macular degeneration. In: Clinical Ophthalmology. 2013 ; Vol. 7. pp. 1987-1993.

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abstract = "Purpose: To ascertain whether single nucleotide polymorphisms (SNPs) in the Vascular Endothelial Growth factor (VEGFA), Complement Factor H (CFH), and LOC387715 genes could predict outcome to anti-VEGF therapy for patients with age related macular degeneration (AMD). Methods: Patients with {"}wet{"} AMD were identified by chart review. Baseline optical coherence tomography (OCT) and visual acuity (VA) data, and at least 6 months of clinical follow up after 3 initial monthly injections of bevacizumab or ranibizumab were required for inclusion. Based on OCT and VA, patients were categorized into two possible clinical outcomes: (a) responders and (b) non-responders. DNA was extracted from saliva and genotyped for candidate SNPs in the VEGFA, LOC387715, and CFH genes. Clinical outcomes were statistically compared to patient genotypes. Results: 101 patients were recruited, and one eye from each patient was included in the analysis. 97% of samples were successfully genotyped for all SNPs. We found a statistically significant association between the LOC387715 A69S TT genotype and outcome based on OCT. Conclusion: Genetic variation may be associated with outcome in patients receiving anti-VEGF therapy.",

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