A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals

Exing Wang, Daniel J. Meier, Ruben M. Sandoval, Vanessa E. Von Hendy-Willson, Barrak M. Pressler, Robert M. Bunch, Mouhamad Alloosh, Michael Sturek, George J. Schwartz, Bruce Molitoris

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Measurement of the glomerular filtration rate (GFR) is the gold standard for precise assessment of kidney function. A rapid, point-of-care determination of the GFR may provide advantages in the clinical setting over currently available assays. Here we demonstrate a proof of principle for such an approach in a pig and dogs, two species that approximate the vascular access and GFR results expected in humans. In both animal models, a sub-millimeter optical fiber that delivered excitation light and collected fluorescent emissions was inserted into a peripheral vein (dog) or central venous access (pig) by means of commercial intravenous catheters. A mixture of fluorescent chimeras of a small freely filterable reporter and large non-filterable plasma volume marker were infused as a bolus, excited by light-emitting diodes, and the in vivo signals detected and quantified by photomultiplier tubes in both species in less than 60 min. Concurrent standardized 6-h iohexol plasma kidney clearances validated the accuracy of our results for both physiologic and a chronic kidney disease setting. Thus, our ratiometric technique allows for both measurement of plasma vascular volume and highly accurate real-time GFR determinations, enabling clinical decision making in real time.

Original languageEnglish
Pages (from-to)112-117
Number of pages6
JournalKidney International
Volume81
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Point-of-Care Systems
Glomerular Filtration Rate
Fluorescence
Plasma Volume
Blood Vessels
Swine
Dogs
Iohexol
Kidney
Optical Fibers
Light
Chronic Renal Insufficiency
Veins
Catheters
Animal Models

Keywords

  • acute kidney injury
  • chronic kidney disease
  • glomerular filtration rate

ASJC Scopus subject areas

  • Nephrology

Cite this

A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals. / Wang, Exing; Meier, Daniel J.; Sandoval, Ruben M.; Von Hendy-Willson, Vanessa E.; Pressler, Barrak M.; Bunch, Robert M.; Alloosh, Mouhamad; Sturek, Michael; Schwartz, George J.; Molitoris, Bruce.

In: Kidney International, Vol. 81, No. 1, 01.01.2012, p. 112-117.

Research output: Contribution to journalArticle

Wang, Exing ; Meier, Daniel J. ; Sandoval, Ruben M. ; Von Hendy-Willson, Vanessa E. ; Pressler, Barrak M. ; Bunch, Robert M. ; Alloosh, Mouhamad ; Sturek, Michael ; Schwartz, George J. ; Molitoris, Bruce. / A portable fiberoptic ratiometric fluorescence analyzer provides rapid point-of-care determination of glomerular filtration rate in large animals. In: Kidney International. 2012 ; Vol. 81, No. 1. pp. 112-117.
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