A positive involvement of RecQL4 in UV-induced S-phase arrest

Su Jung Park, Young Ju Lee, Brian D. Beck, Suk Hee Lee

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

RecQL4 belongs to a family of conserved RECQ helicases that are important in maintaining chromosomal integrity. Human patients lacking RecQL4 showed extreme sensitivity to UV and oxidation damage, suggesting that RecQL4 is involved in the damage signaling and/or repair. Here we show that human mutant cells lacking RecQL4 were defective in UV-induced S-phase arrest, whereas cells defective in bloom syndrome protein (BLM), another member of RecQ family exhibited a normal S-phase arrest following UV irradiation. In keeping with this, a targeted inhibition of RecQL4 expression in human 293 cells showed a defect in inducing S-phase (replication) arrest following UV treatment. Human mutant cells lacking RecQL4 protein were also defective in inducing S-phase arrest following hydroxyurea treatment. Together, our results suggest that RecQL4 may have a unique role in replication fork arrest, which may not be shared with other members of RecQ family such as BLM.

Original languageEnglish (US)
Pages (from-to)696-703
Number of pages8
JournalDNA and Cell Biology
Volume25
Issue number12
DOIs
StatePublished - Dec 1 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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    Park, S. J., Lee, Y. J., Beck, B. D., & Lee, S. H. (2006). A positive involvement of RecQL4 in UV-induced S-phase arrest. DNA and Cell Biology, 25(12), 696-703. https://doi.org/10.1089/dna.2006.25.696