A potential for tissue restrictive gene therapy in renal cell carcinoma using MN/CA IX promoter

Yen Chuan Ou, Thomas A. Gardner, Chinghaj Kao, Haiyen E. Zhau, Leland W. Chung

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background: The expression of the MN/carbonic anhydrase IX (CA IX) protein is detected in renal cell carcinoma (RCC). A tumor-specific MN promoter was designed and evaluated. Materials and Methods: Western blot, reverse transcription-PCR and Northern blot for MN expression were performed on Hela cells, LNCaP and four human RCC cell lines. The MN promoter (554 bp) was cloned into pGL3 luciferase reporter vectors and their activities were tested. Results: Hela cells, SKRC-31 and SKRC-38 expressed high levels of MN RNA and MN protein. The MN promoter has 5- to 10-fold higher activity in MN-positive cell lines than in MN-negative cell lines. It could enhance the activity of the basic SV40 early promoter (6 to 25 fold) in MN-positive cells but not in MN-negative cells. Conclusion: MN expression in RCC may derive from altered expression of transcriptional factor(s), which binds to the MN enhancer element, and can be used for tumor specific toxic gene therapy for RCC.

Original languageEnglish (US)
Pages (from-to)881-886
Number of pages6
JournalAnticancer Research
Volume25
Issue number2 A
StatePublished - Mar 1 2005

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Keywords

  • Carbonic anhydrase IX (CA IX)
  • Gene therapy
  • MN protein
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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