A potential role for astrocytes in mediating the antiepileptic actions of furosemide in vitro

Nicholas Barbaro, D. K. Takahashi, S. C. Baraban

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Epileptic seizures are characterized by abnormal electrical discharge. In previous studies we established a powerful antiepileptic action for a commonly used diuretic (furosemide). However, it remains unclear precisely how furosemide terminates abnormal electrical discharges. To address this issue, we performed in vitro experiments to examine conditions where furosemide exerts antiepileptic activity and patch-clamp studies to analyze the effect of furosemide on neuronal membrane properties, synaptic function and inward potassium current. Furosemide was not found to alter synaptic field responses, excitatory postsynaptic currents or intrinsic membrane properties of principal hippocampal neurons. Our in vitro studies indicate that furosemide does not abolish spontaneous epileptiform bursting during co-application of Ba 2+ or Cs + ions (to block inwardly rectifying potassium channels). Our patch-clamp data indicate that furosemide enhances the function of astrocytic, but not neuronal, inward potassium channels and that this modulation may be required for its antiepileptic activity. Although a variety of antiepileptic drugs are already available, none of these compounds selectively target astrocytes while preserving synaptic/neuronal function. Thus, furosemide-mediated modulation of inward potassium current (on astrocytes) represents a new target for control of abnormal electrical discharge in the CNS.

Original languageEnglish (US)
Pages (from-to)655-663
Number of pages9
JournalNeuroscience
Volume128
Issue number3
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Furosemide
Astrocytes
Anticonvulsants
Potassium
Inwardly Rectifying Potassium Channel
Synaptic Membranes
In Vitro Techniques
Excitatory Postsynaptic Potentials
Potassium Channels
Diuretics
Epilepsy
Ions
Neurons
Membranes

Keywords

  • astrocyte
  • epilepsy
  • hippocampus
  • non-synaptic
  • patch-clamp
  • seizure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A potential role for astrocytes in mediating the antiepileptic actions of furosemide in vitro. / Barbaro, Nicholas; Takahashi, D. K.; Baraban, S. C.

In: Neuroscience, Vol. 128, No. 3, 2004, p. 655-663.

Research output: Contribution to journalArticle

Barbaro, Nicholas ; Takahashi, D. K. ; Baraban, S. C. / A potential role for astrocytes in mediating the antiepileptic actions of furosemide in vitro. In: Neuroscience. 2004 ; Vol. 128, No. 3. pp. 655-663.
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