A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations

V. H. Gattone, N. S. Fineberg, A. P. Evan

Research output: Contribution to journalArticle

Abstract

Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

Original languageEnglish (US)
Pages (from-to)551-554
Number of pages4
JournalJournal of Submicroscopic Cytology
Volume17
Issue number4
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. / Gattone, V. H.; Fineberg, N. S.; Evan, A. P.

In: Journal of Submicroscopic Cytology, Vol. 17, No. 4, 01.12.1985, p. 551-554.

Research output: Contribution to journalArticle

@article{ca7520deea9742f5a3faab9901506e6d,
title = "A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations",
abstract = "Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.",
author = "Gattone, {V. H.} and Fineberg, {N. S.} and Evan, {A. P.}",
year = "1985",
month = "12",
day = "1",
language = "English (US)",
volume = "17",
pages = "551--554",
journal = "Journal of Submicroscopic Cytology",
number = "4",

}

TY - JOUR

T1 - A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations

AU - Gattone, V. H.

AU - Fineberg, N. S.

AU - Evan, A. P.

PY - 1985/12/1

Y1 - 1985/12/1

N2 - Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

AB - Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

UR - http://www.scopus.com/inward/record.url?scp=0022217449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022217449&partnerID=8YFLogxK

M3 - Article

C2 - 4078947

AN - SCOPUS:0022217449

VL - 17

SP - 551

EP - 554

JO - Journal of Submicroscopic Cytology

JF - Journal of Submicroscopic Cytology

IS - 4

ER -