A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations

V. H. Gattone, N. S. Fineberg, Andrew Evan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

Original languageEnglish (US)
Title of host publicationJournal of Submicroscopic Cytology
Pages551-554
Number of pages4
Volume17
Edition4
StatePublished - 1985
Externally publishedYes

Fingerprint

Smooth Muscle Myocytes
Electrons
Microvessels
Electron Scanning Microscopy
Cell Count

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Gattone, V. H., Fineberg, N. S., & Evan, A. (1985). A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. In Journal of Submicroscopic Cytology (4 ed., Vol. 17, pp. 551-554)

A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. / Gattone, V. H.; Fineberg, N. S.; Evan, Andrew.

Journal of Submicroscopic Cytology. Vol. 17 4. ed. 1985. p. 551-554.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gattone, VH, Fineberg, NS & Evan, A 1985, A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. in Journal of Submicroscopic Cytology. 4 edn, vol. 17, pp. 551-554.
Gattone VH, Fineberg NS, Evan A. A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. In Journal of Submicroscopic Cytology. 4 ed. Vol. 17. 1985. p. 551-554
Gattone, V. H. ; Fineberg, N. S. ; Evan, Andrew. / A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations. Journal of Submicroscopic Cytology. Vol. 17 4. ed. 1985. pp. 551-554
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