### Abstract

Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

Original language | English (US) |
---|---|

Title of host publication | Journal of Submicroscopic Cytology |

Pages | 551-554 |

Number of pages | 4 |

Volume | 17 |

Edition | 4 |

State | Published - 1985 |

Externally published | Yes |

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### ASJC Scopus subject areas

- Anatomy
- Histology

### Cite this

*Journal of Submicroscopic Cytology*(4 ed., Vol. 17, pp. 551-554)

**A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations.** / Gattone, V. H.; Fineberg, N. S.; Evan, Andrew.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

*Journal of Submicroscopic Cytology.*4 edn, vol. 17, pp. 551-554.

}

TY - CHAP

T1 - A proposed technique for the morphometric analysis of arteriolar smooth muscle cells from scanning electron microscopic preparations

AU - Gattone, V. H.

AU - Fineberg, N. S.

AU - Evan, Andrew

PY - 1985

Y1 - 1985

N2 - Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

AB - Morphometric methods are developed which allow the determination of smooth muscle cell (SMC) features from microvasculature prepared for scanning electron microscopy (SEM). In vessels so prepared, the SMCs are visible as either tapered ends (T) or complete wraps (CW) around the visible vessel. Formulas were developed so that these features could be used to determine the number of cells (C) in a vessel segment and the number of times the smooth muscle cells wrap around the vessel (W/C). Models were constructed to simulate such vessel preparations to test these formulas. From the two pieces of data (C and W/C) so determined, other SMC parameters could be calculated such as: SMC density along the vessel, SMC length and vessel surface area covered by a SMC. Therefore, it is evident that SMC data can be determined from microvessels prepared for SEM. This data would allow a more complete understanding of SMCs and their changes in normal and diseased states.

UR - http://www.scopus.com/inward/record.url?scp=0022217449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022217449&partnerID=8YFLogxK

M3 - Chapter

VL - 17

SP - 551

EP - 554

BT - Journal of Submicroscopic Cytology

ER -