'A' protein of amyloidosis. Isolation of a cross reacting component from serum by affinity chromatography

Merrill Benson, M. Skinner, J. Lian, A. S. Cohen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A nonimmunoglobulin protein (A protein) has been isolated from amyloidotic tissue of secondary type. Antisera prepared to this protein identified a cross reacting substance in the sera of patients with secondary amyloidosis. Sera from 70 persons with amyloidosis, 120 normal adults, 20 aged persons, and 97 patients with chronic diseases were tested for this substance. One hundred percent of secondary amyloid sera had amounts of amyloid serum component detectable by double diffusion in agar, whereas only 19% of primary amyloid sera were positive. Approximately 60% of rheumatoid sera as well as 60% of sera from aged individuals were positive. Only 3% of normal blood donors had detectable amounts of this circulating substance. Isolation of the serum component by affinity chromatography and partial characterization have shown that it is an α globulin with a molecular weight of 100,000-120,000, that it is not related antigenically to immunoglobulin or amyloid P component, and that it has an amino acid analysis that is markedly different from tissue A protein. The possible participation of this substance in the genesis of amyloid is discussed.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalArthritis and Rheumatism
Volume18
Issue number4
StatePublished - 1975
Externally publishedYes

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Amyloidosis
Affinity Chromatography
Serum
Proteins
Amyloid
Serum Amyloid P-Component
Globulins
Blood Donors
Agar
Immunoglobulins
Immune Sera
Chronic Disease
Molecular Weight
Amino Acids

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

'A' protein of amyloidosis. Isolation of a cross reacting component from serum by affinity chromatography. / Benson, Merrill; Skinner, M.; Lian, J.; Cohen, A. S.

In: Arthritis and Rheumatism, Vol. 18, No. 4, 1975, p. 315-322.

Research output: Contribution to journalArticle

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