A proteomic workflow for discovery of serum carrier protein-bound biomarker candidates of alcohol abuse using LC-MS/MS

Xianyin Lai, Suthat Liangpunsakul, David Crabb, Heather N. Ringham, Frank Witzmann

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The diagnosis and care of patients with alcohol abuse and dependence is hampered by a lack of sensitive and specific screening and monitoring tests. Proteomics is a good approach to search for biomarkers of alcohol abuse. Serum carrier protein-bound proteins have attracted significant interest because they remain a relatively un-mined region of the proteome. In the present study, a proteomic workflow including LC-MS/MS with enrichment of serum carrier protein-bound biomarkers technique was applied to profile the changes in quality and quantity of serum carrier protein-bound proteins for the discovery of novel biomarker candidates of alcohol abuse. In total, 311 proteins identified with high confidence were discovered to be bound to serum carrier proteins. Complement isoforms, Ig fragments, and apolipoprotein family proteins are the main serum carrier-bound proteins. Protein quantification analysis with and without concern as to gender revealed that gender is a critical consideration for biomarker development in alcohol abuse. Identified proteins not previously associated with alcohol abuse include gelsolin, selenoprotein P, serotransferrin, tetranectin, hemopexin, histidine-rich glycoprotein, plasma kallikrein, and vitronectin. Altered abundance of these proteins suggests that they may be potential novel biomarkers for alcohol abuse.

Original languageEnglish
Pages (from-to)2207-2214
Number of pages8
JournalElectrophoresis
Volume30
Issue number12
DOIs
StatePublished - Jun 2009

Fingerprint

Workflow
Biomarkers
Proteomics
Alcoholism
Blood Proteins
Carrier Proteins
Alcohols
Proteins
Selenoprotein P
Hemopexin
Plasma Kallikrein
Gelsolin
Vitronectin
Apolipoproteins
Proteome
Transferrin
Patient Care
Protein Isoforms
Screening
Monitoring

Keywords

  • Alcohol abuse
  • Biomarker
  • MS/MS
  • Proteomics
  • Serum carrier protein

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

A proteomic workflow for discovery of serum carrier protein-bound biomarker candidates of alcohol abuse using LC-MS/MS. / Lai, Xianyin; Liangpunsakul, Suthat; Crabb, David; Ringham, Heather N.; Witzmann, Frank.

In: Electrophoresis, Vol. 30, No. 12, 06.2009, p. 2207-2214.

Research output: Contribution to journalArticle

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