A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity

Takasumi Shimomoto, Leonard B. Collins, Xianwen Yi, Darcy W. Holley, Zhenfa Zhang, Xu Tian, Koji Uchida, Chunguang Wang, Sohvi Hörkkö, Monte S. Willis, Avram Gold, Scott J. Bultman, Jun Nakamura

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Atherosclerosis is widely accepted to be a chronic inflammatory disease, and the immunological response to the accumulation of LDL is believed to play a critical role in the development of this disease. 1,4-Dihydropyridine-type MAA-adducted LDL has been implicated in atherosclerosis. Here, we have demonstrated that pure MAA-modified residues can be chemically conjugated to large proteins without by-product contamination. Using this pure antigen, we established a purified MAA-ELISA, with which a marked increase in anti-MAA antibody titer was determined at a very early stage of atherosclerosis in 3-month ApoE-/-mice fed with a normal diet. Our methods of Nå -MAA-L-lysine purification and purified antigen-based ELISA will be easily applicable for biomarker-based detection of early stage atherosclerosis in patients, as well as for the development of an adduct-specific Liquid Chromatography/Mass Spectrometry-based quantification of physiological and pathological levels of MAA.

Original languageEnglish (US)
Article numbere0172172
JournalPloS one
Volume12
Issue number2
DOIs
StatePublished - Feb 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Shimomoto, T., Collins, L. B., Yi, X., Holley, D. W., Zhang, Z., Tian, X., Uchida, K., Wang, C., Hörkkö, S., Willis, M. S., Gold, A., Bultman, S. J., & Nakamura, J. (2017). A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity. PloS one, 12(2), [e0172172]. https://doi.org/10.1371/journal.pone.0172172