A putative role for psoriasin in breast tumor progression

Ian Krop, Annette März, Hanna Carlsson, Xiaochun Li, Noga Bloushtain-Qimron, Min Hu, Rebecca Gelman, Michael S. Sabel, Stuart Schnitt, Sridhar Ramaswamy, Celina G. Kleer, Charlotta Enerbäck, Kornelia Polyak

Research output: Contribution to journalArticle

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Abstract

Psoriasin (S100A7) was identified as a gene highly expressed in psoriatic keratinocytes and highly and more frequently expressed in ductal carcinoma in situ (DCIS) than in invasive breast carcinomas (IBC), suggesting a potential role in tumor progression. Psoriasin expression is associated with poor prognostic factors in both DCIS and IBC. Several putative functions have been proposed for psoriasin in various disease types, but none of these can fully explain its involvement in breast tumor progression. Here, we show that downregulation of endogenous psoriasin expression via stable short hairpin RNAs in a human IBC cell line (MDA-MB-468) increases cell migration and invasion without influencing cell proliferation and survival in vitro but inhibits tumor growth in vivo. These seemingly paradoxical results are potentially explained by the dramatic up-regulation and down-regulation of matrix metalloproteinase-13 and vascular endothelial growth factor (VEGF), respectively, observed in cells with decreased psoriasin levels compared with controls. Correlating with this, high psoriasin expression in human IBC is associated with increased angiogenesis and worse clinical outcome, and psoriasin mRNA levels are coordinately regulated with VEGF and other genes related to hypoxia and mitochondrial reactive oxygen species (ROS). Based on these results, we propose that psoriasin may play a role in breast tumor progression by promoting angiogenesis and enhancing the selection for cells that overcome its anti-invasive function. This hypothesis may explain why psoriasin expression is highest in high-grade and/or estrogen receptor-negative tumors, as these are associated with increased hypoxia and ROS, a setting in which the angiogenic effects of psoriasin are most important.

Original languageEnglish (US)
Pages (from-to)11326-11334
Number of pages9
JournalCancer Research
Volume65
Issue number24
DOIs
StatePublished - Dec 15 2005
Externally publishedYes

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Breast Neoplasms
Carcinoma, Intraductal, Noninfiltrating
Vascular Endothelial Growth Factor A
Reactive Oxygen Species
Down-Regulation
Matrix Metalloproteinase 13
Neoplasms
Keratinocytes
Estrogen Receptors
Small Interfering RNA
Genes
Cell Movement
Cell Survival
Up-Regulation
Cell Proliferation
Cell Line
Messenger RNA
Growth
Hypoxia

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Krop, I., März, A., Carlsson, H., Li, X., Bloushtain-Qimron, N., Hu, M., ... Polyak, K. (2005). A putative role for psoriasin in breast tumor progression. Cancer Research, 65(24), 11326-11334. https://doi.org/10.1158/0008-5472.CAN-05-1523

A putative role for psoriasin in breast tumor progression. / Krop, Ian; März, Annette; Carlsson, Hanna; Li, Xiaochun; Bloushtain-Qimron, Noga; Hu, Min; Gelman, Rebecca; Sabel, Michael S.; Schnitt, Stuart; Ramaswamy, Sridhar; Kleer, Celina G.; Enerbäck, Charlotta; Polyak, Kornelia.

In: Cancer Research, Vol. 65, No. 24, 15.12.2005, p. 11326-11334.

Research output: Contribution to journalArticle

Krop, I, März, A, Carlsson, H, Li, X, Bloushtain-Qimron, N, Hu, M, Gelman, R, Sabel, MS, Schnitt, S, Ramaswamy, S, Kleer, CG, Enerbäck, C & Polyak, K 2005, 'A putative role for psoriasin in breast tumor progression', Cancer Research, vol. 65, no. 24, pp. 11326-11334. https://doi.org/10.1158/0008-5472.CAN-05-1523
Krop I, März A, Carlsson H, Li X, Bloushtain-Qimron N, Hu M et al. A putative role for psoriasin in breast tumor progression. Cancer Research. 2005 Dec 15;65(24):11326-11334. https://doi.org/10.1158/0008-5472.CAN-05-1523
Krop, Ian ; März, Annette ; Carlsson, Hanna ; Li, Xiaochun ; Bloushtain-Qimron, Noga ; Hu, Min ; Gelman, Rebecca ; Sabel, Michael S. ; Schnitt, Stuart ; Ramaswamy, Sridhar ; Kleer, Celina G. ; Enerbäck, Charlotta ; Polyak, Kornelia. / A putative role for psoriasin in breast tumor progression. In: Cancer Research. 2005 ; Vol. 65, No. 24. pp. 11326-11334.
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abstract = "Psoriasin (S100A7) was identified as a gene highly expressed in psoriatic keratinocytes and highly and more frequently expressed in ductal carcinoma in situ (DCIS) than in invasive breast carcinomas (IBC), suggesting a potential role in tumor progression. Psoriasin expression is associated with poor prognostic factors in both DCIS and IBC. Several putative functions have been proposed for psoriasin in various disease types, but none of these can fully explain its involvement in breast tumor progression. Here, we show that downregulation of endogenous psoriasin expression via stable short hairpin RNAs in a human IBC cell line (MDA-MB-468) increases cell migration and invasion without influencing cell proliferation and survival in vitro but inhibits tumor growth in vivo. These seemingly paradoxical results are potentially explained by the dramatic up-regulation and down-regulation of matrix metalloproteinase-13 and vascular endothelial growth factor (VEGF), respectively, observed in cells with decreased psoriasin levels compared with controls. Correlating with this, high psoriasin expression in human IBC is associated with increased angiogenesis and worse clinical outcome, and psoriasin mRNA levels are coordinately regulated with VEGF and other genes related to hypoxia and mitochondrial reactive oxygen species (ROS). Based on these results, we propose that psoriasin may play a role in breast tumor progression by promoting angiogenesis and enhancing the selection for cells that overcome its anti-invasive function. This hypothesis may explain why psoriasin expression is highest in high-grade and/or estrogen receptor-negative tumors, as these are associated with increased hypoxia and ROS, a setting in which the angiogenic effects of psoriasin are most important.",
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AU - März, Annette

AU - Carlsson, Hanna

AU - Li, Xiaochun

AU - Bloushtain-Qimron, Noga

AU - Hu, Min

AU - Gelman, Rebecca

AU - Sabel, Michael S.

AU - Schnitt, Stuart

AU - Ramaswamy, Sridhar

AU - Kleer, Celina G.

AU - Enerbäck, Charlotta

AU - Polyak, Kornelia

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