A rapid and efficient, nonradioactive method for screening recombinant DNA libraries

L. Amaravadi, Michael King

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this report we present a rapid and inexpensive PCR-based method to screen recombinant DNA libraries. The efficiency of this method was demonstrated by the isolation of clones of interest from three different libraries using different vector systems. This method is nonradioactive and makes it easier to handle a large number of samples since there is no need for DNA extraction. The advantages and applications of the method are discussed.

Original languageEnglish
JournalBioTechniques
Volume16
Issue number1
StatePublished - 1994

Fingerprint

Recombinant DNA
Gene Library
Screening
DNA
Libraries
Clone Cells
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

A rapid and efficient, nonradioactive method for screening recombinant DNA libraries. / Amaravadi, L.; King, Michael.

In: BioTechniques, Vol. 16, No. 1, 1994.

Research output: Contribution to journalArticle

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