A rapid and simple HPLC microassay for biogenic amines in discrete brain regions

Bruce A. Donzanti, Bryan K. Yamamoto

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A rapid microassay is described for the measurement of biogenic amines using an isocratic HPLC system with electrochemical detection. Catecholomines, indoleamines and their major metabolites were extracted with 150 μl of perchloric acid from brain tissue punches (<250 μg) using a simple one-step sample preparation method. These compounds were separated on a short (80 mm) column with 3 μm particle size packing, and electrochemically detected within a total run time of less than 6 minutes. Detection limit sensitivity was approximately 2-5 pg. This method, detailed in an easy-to-follow description, reduces assay time, minimizes the possibility for errors, maximizes efficiency, and requires only standard HPLC equipment and supplies.

Original languageEnglish (US)
Pages (from-to)795-799
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume30
Issue number3
DOIs
StatePublished - Jul 1988

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Keywords

  • Brain
  • Catecholamines
  • HPLC
  • Indoleamines

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

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