A rapid and simple HPLC microassay for biogenic amines in discrete brain regions

Bruce A. Donzanti, Bryan Yamamoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A rapid microassay is described for the measurement of biogenic amines using an isocratic HPLC system with electrochemical detection. Catecholomines, indoleamines and their major metabolites were extracted with 150 μl of perchloric acid from brain tissue punches (<250 μg) using a simple one-step sample preparation method. These compounds were separated on a short (80 mm) column with 3 μm particle size packing, and electrochemically detected within a total run time of less than 6 minutes. Detection limit sensitivity was approximately 2-5 pg. This method, detailed in an easy-to-follow description, reduces assay time, minimizes the possibility for errors, maximizes efficiency, and requires only standard HPLC equipment and supplies.

Original languageEnglish (US)
Pages (from-to)795-799
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume30
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Biogenic Amines
Metabolites
Assays
Brain
Particle size
High Pressure Liquid Chromatography
Tissue
Particle Size
Limit of Detection
Equipment and Supplies
Perchloric Acid

Keywords

  • Brain
  • Catecholamines
  • HPLC
  • Indoleamines

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

A rapid and simple HPLC microassay for biogenic amines in discrete brain regions. / Donzanti, Bruce A.; Yamamoto, Bryan.

In: Pharmacology, Biochemistry and Behavior, Vol. 30, No. 3, 1988, p. 795-799.

Research output: Contribution to journalArticle

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