A rat head holder for simultaneous scanning of two rats in small animal PET scanners: Design, construction, feasibility testing and kinetic validation

Tee Ean Cheng, Karmen Yoder, Marc D. Normandin, Shannon L. Risacher, Alexander K. Converse, Joseph A. Hampel, Michael A. Miller, Evan D. Morris

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To reduce imaging costs, we designed a head holder for scanning two rats simultaneously in small animal PET scanners. Our goals were (i) to maintain high sensitivity and (ii) to minimize repositioning error between scans. Methods: A semi-stereotaxic dual rat head holder was designed and constructed for dual rat scanning in our IndyPET-II scanner and the commercial microPET P4. It was also used for single rat scanning in a small-bore, high-resolution animal scanner ("ISAP"). Positional repeatability was validated via multiple [11C]Raclopride scans of a single rat on different days. Accuracy of repositioning was determined by visual comparison of images, and by metrics derived through image alignment. Kinetic validation was assessed via analysis of [18F]Fluorodeoxyglucose ([18F]FDG) dynamic PET studies of six rats. Each rat was scanned twice: once individually, with brain positioned at the center of field of view (CFOV), and once with a partner, with brain away from CFOV. Both rats were injected with FDG during each dual rat session. Patlak uptake constants (Ki) were calculated from whole brain images. Effects of attenuation and scatter correction on single versus dual scan images were explored. Results: Image comparison and alignment metrics indicated excellent repositioning of rats. Scaled time-activity-curves from single and dual rat scans were indistinguishable. Average single and dual scan Ki values differed by only 6.3 ± 7.5%. Conclusion: Dual rat scanning in a semi-stereotaxic holder is practical for economical small animal scanning and does not compromise kinetic accuracy of [18F]FDG dynamic scan data.

Original languageEnglish
Pages (from-to)24-33
Number of pages10
JournalJournal of Neuroscience Methods
Volume176
Issue number1
DOIs
StatePublished - Jan 15 2009

Fingerprint

Head
Fluorodeoxyglucose F18
Brain
Raclopride
Costs and Cost Analysis

Keywords

  • Attenuation correction
  • Multiple rat scanning
  • Patlak plot
  • Positron emission tomography
  • Rat holder
  • Scatter correction
  • Simultaneous scanning
  • Small animal scanning

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A rat head holder for simultaneous scanning of two rats in small animal PET scanners : Design, construction, feasibility testing and kinetic validation. / Cheng, Tee Ean; Yoder, Karmen; Normandin, Marc D.; Risacher, Shannon L.; Converse, Alexander K.; Hampel, Joseph A.; Miller, Michael A.; Morris, Evan D.

In: Journal of Neuroscience Methods, Vol. 176, No. 1, 15.01.2009, p. 24-33.

Research output: Contribution to journalArticle

Cheng, Tee Ean ; Yoder, Karmen ; Normandin, Marc D. ; Risacher, Shannon L. ; Converse, Alexander K. ; Hampel, Joseph A. ; Miller, Michael A. ; Morris, Evan D. / A rat head holder for simultaneous scanning of two rats in small animal PET scanners : Design, construction, feasibility testing and kinetic validation. In: Journal of Neuroscience Methods. 2009 ; Vol. 176, No. 1. pp. 24-33.
@article{c032dae6c5e74e7c8601b14df17056c1,
title = "A rat head holder for simultaneous scanning of two rats in small animal PET scanners: Design, construction, feasibility testing and kinetic validation",
abstract = "To reduce imaging costs, we designed a head holder for scanning two rats simultaneously in small animal PET scanners. Our goals were (i) to maintain high sensitivity and (ii) to minimize repositioning error between scans. Methods: A semi-stereotaxic dual rat head holder was designed and constructed for dual rat scanning in our IndyPET-II scanner and the commercial microPET P4. It was also used for single rat scanning in a small-bore, high-resolution animal scanner ({"}ISAP{"}). Positional repeatability was validated via multiple [11C]Raclopride scans of a single rat on different days. Accuracy of repositioning was determined by visual comparison of images, and by metrics derived through image alignment. Kinetic validation was assessed via analysis of [18F]Fluorodeoxyglucose ([18F]FDG) dynamic PET studies of six rats. Each rat was scanned twice: once individually, with brain positioned at the center of field of view (CFOV), and once with a partner, with brain away from CFOV. Both rats were injected with FDG during each dual rat session. Patlak uptake constants (Ki) were calculated from whole brain images. Effects of attenuation and scatter correction on single versus dual scan images were explored. Results: Image comparison and alignment metrics indicated excellent repositioning of rats. Scaled time-activity-curves from single and dual rat scans were indistinguishable. Average single and dual scan Ki values differed by only 6.3 ± 7.5{\%}. Conclusion: Dual rat scanning in a semi-stereotaxic holder is practical for economical small animal scanning and does not compromise kinetic accuracy of [18F]FDG dynamic scan data.",
keywords = "Attenuation correction, Multiple rat scanning, Patlak plot, Positron emission tomography, Rat holder, Scatter correction, Simultaneous scanning, Small animal scanning",
author = "Cheng, {Tee Ean} and Karmen Yoder and Normandin, {Marc D.} and Risacher, {Shannon L.} and Converse, {Alexander K.} and Hampel, {Joseph A.} and Miller, {Michael A.} and Morris, {Evan D.}",
year = "2009",
month = "1",
day = "15",
doi = "10.1016/j.jneumeth.2008.08.031",
language = "English",
volume = "176",
pages = "24--33",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A rat head holder for simultaneous scanning of two rats in small animal PET scanners

T2 - Design, construction, feasibility testing and kinetic validation

AU - Cheng, Tee Ean

AU - Yoder, Karmen

AU - Normandin, Marc D.

AU - Risacher, Shannon L.

AU - Converse, Alexander K.

AU - Hampel, Joseph A.

AU - Miller, Michael A.

AU - Morris, Evan D.

PY - 2009/1/15

Y1 - 2009/1/15

N2 - To reduce imaging costs, we designed a head holder for scanning two rats simultaneously in small animal PET scanners. Our goals were (i) to maintain high sensitivity and (ii) to minimize repositioning error between scans. Methods: A semi-stereotaxic dual rat head holder was designed and constructed for dual rat scanning in our IndyPET-II scanner and the commercial microPET P4. It was also used for single rat scanning in a small-bore, high-resolution animal scanner ("ISAP"). Positional repeatability was validated via multiple [11C]Raclopride scans of a single rat on different days. Accuracy of repositioning was determined by visual comparison of images, and by metrics derived through image alignment. Kinetic validation was assessed via analysis of [18F]Fluorodeoxyglucose ([18F]FDG) dynamic PET studies of six rats. Each rat was scanned twice: once individually, with brain positioned at the center of field of view (CFOV), and once with a partner, with brain away from CFOV. Both rats were injected with FDG during each dual rat session. Patlak uptake constants (Ki) were calculated from whole brain images. Effects of attenuation and scatter correction on single versus dual scan images were explored. Results: Image comparison and alignment metrics indicated excellent repositioning of rats. Scaled time-activity-curves from single and dual rat scans were indistinguishable. Average single and dual scan Ki values differed by only 6.3 ± 7.5%. Conclusion: Dual rat scanning in a semi-stereotaxic holder is practical for economical small animal scanning and does not compromise kinetic accuracy of [18F]FDG dynamic scan data.

AB - To reduce imaging costs, we designed a head holder for scanning two rats simultaneously in small animal PET scanners. Our goals were (i) to maintain high sensitivity and (ii) to minimize repositioning error between scans. Methods: A semi-stereotaxic dual rat head holder was designed and constructed for dual rat scanning in our IndyPET-II scanner and the commercial microPET P4. It was also used for single rat scanning in a small-bore, high-resolution animal scanner ("ISAP"). Positional repeatability was validated via multiple [11C]Raclopride scans of a single rat on different days. Accuracy of repositioning was determined by visual comparison of images, and by metrics derived through image alignment. Kinetic validation was assessed via analysis of [18F]Fluorodeoxyglucose ([18F]FDG) dynamic PET studies of six rats. Each rat was scanned twice: once individually, with brain positioned at the center of field of view (CFOV), and once with a partner, with brain away from CFOV. Both rats were injected with FDG during each dual rat session. Patlak uptake constants (Ki) were calculated from whole brain images. Effects of attenuation and scatter correction on single versus dual scan images were explored. Results: Image comparison and alignment metrics indicated excellent repositioning of rats. Scaled time-activity-curves from single and dual rat scans were indistinguishable. Average single and dual scan Ki values differed by only 6.3 ± 7.5%. Conclusion: Dual rat scanning in a semi-stereotaxic holder is practical for economical small animal scanning and does not compromise kinetic accuracy of [18F]FDG dynamic scan data.

KW - Attenuation correction

KW - Multiple rat scanning

KW - Patlak plot

KW - Positron emission tomography

KW - Rat holder

KW - Scatter correction

KW - Simultaneous scanning

KW - Small animal scanning

UR - http://www.scopus.com/inward/record.url?scp=54049102352&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54049102352&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2008.08.031

DO - 10.1016/j.jneumeth.2008.08.031

M3 - Article

C2 - 18824025

AN - SCOPUS:54049102352

VL - 176

SP - 24

EP - 33

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1

ER -