A recombinant human fibronectin fragment facilitates retroviral mediated gene transfer into human hematopoietic progenitor cells

Parmesh Dutt, Helmut Hanenberg, Terry Vik, David A. Williams, Mervin C. Yoder

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Gene transfer into hematopoietic progenitor cells is increased when these cells are adherent to the carboxy-terminal chymotryptic fragment of human plasma fibronectin (FN30/35) containing the heparin binding domain (KBD) and the alternatively spliced type three connecting segment (IIICS) region. We report herein the production of a recombinant human fibronectin fragment comprised of the HBD and IIICS regions expressed by Sf9 insect cells following recombinant baculovirus infection. The resulting isolated peptide, HuBacFN, facilitated gene transfer into human hematopoietic cell lines and primary human hematopoietic progenitors to a level achieved with the purified human plasma FN30/35 peptide.

Original languageEnglish (US)
Pages (from-to)909-917
Number of pages9
JournalBiochemistry and Molecular Biology International
Volume42
Issue number5
DOIs
StatePublished - Aug 1997

Keywords

  • Fibronectin
  • Gene transfer
  • Hematopoiesis
  • Progenitor cell

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

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