A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay

A. L. Aloia, L. Duffy, V. Pak, K. E. Lee, S. Sanchez-Martinez, D. Derse, G. Heidecker, K. Cornetta, A. Rein

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+ L- or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+ L- assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalGene Therapy
Volume20
Issue number2
DOIs
StatePublished - Feb 1 2013

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Keywords

  • Gene-therapy vectors
  • Infectivity assay
  • Replication-competent gammaretrovirus

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Aloia, A. L., Duffy, L., Pak, V., Lee, K. E., Sanchez-Martinez, S., Derse, D., Heidecker, G., Cornetta, K., & Rein, A. (2013). A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay. Gene Therapy, 20(2), 169-176. https://doi.org/10.1038/gt.2012.18