A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines.

Kenneth Cornetta, D. Berebitsky, M. Behnia, Christie Orschell, Edward Srour, G. W. Sledge

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The use of cytokines to modify antigen expression on syngeneic murine tumor cells has led to immunization of the host from subsequent challenges with the parent tumor cell line. To determine whether this approach can be applied to human malignancies we introduced the human interferon gamma gene (IFN gamma) into the human breast cancer cell lines MDA-MB-435 and MDA-MB-231 using retroviral-mediated gene transfer. Retroviral transfer of the IFN gamma gene was associated with decreased growth, decreased tumor invasiveness, IFN gamma production, and upregulation of MHC antigens. While the MDA-MB-435 produced higher levels of IFN gamma than MDA-MB-231, MHC class I and class II antigens were upregulated in both cell lines. Introduction of this vector into the human leukemia cell line K562 led to increased expression of MHC class I antigens, but not class II. Our findings suggest that expression of interferon gamma in breast cancer cells may lead to increased recognition of breast cancer cells by the host immune system. Furthermore, these data suggest that further development of this approach to cancer immunotherapy is warranted.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalCancer Gene Therapy
Volume1
Issue number2
StatePublished - Jun 1994

Fingerprint

Leukemia
Up-Regulation
Breast Neoplasms
Antigens
Cell Line
Interferon-gamma
Genes
Neoplasms
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Tumor Cell Line
Immunotherapy
human IFNG protein
Immune System
Immunization
Cytokines
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines. / Cornetta, Kenneth; Berebitsky, D.; Behnia, M.; Orschell, Christie; Srour, Edward; Sledge, G. W.

In: Cancer Gene Therapy, Vol. 1, No. 2, 06.1994, p. 91-98.

Research output: Contribution to journalArticle

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