A role for CD8+ T lymphocytes in osteoclast differentiation in vitro

Vanchit John, Janet M. Hock, Lorri L. Short, Andrew L. Glasebrook, Rachelle J Sells Galvin

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Bone loss associated with chronic inflammatory diseases has been attributed to the release of cytokines from T lymphocytes. However, the role oft lymphocyte subsets in the mediation of osteoclast activity has not been extensively studied. Cocultures of murine bone marrow and BALC cells (murine calvarial-derived cell line) were used to study osteoclast differentiation. Murine marrow was left intact or depleted of cells expressing the CD8 or CD4 antigen by immunomagnetic separation and then cocultured with BALC cells in the presence or absence of 1,25-(OH)2D3. Depleting bone marrow of CD4- positive (CD4+) cells did not affect osteoclast differentiation (formation of tartrate-resistant acid phosphatase positive cells with three or more nuclei). However, depletion of CD8-positive (CD8+) cells resulted in a 40% increase in the number of osteoclasts formed. Addition of CD8+ cells to CD8+ cell depleted cocultures via Transwells abolished the stimulatory effects on osteoclast differentiation resulting from CD8+ cell depletion. Neutralizing antibodies to interleukin-4 and transforming growth factor-β did not affect osteoclast differentiation in these cultures. These findings suggest that CD8+ cells may be involved in the regulation of osteoclast differentiation and that this effect is not mediated by interleukin-4 or transforming growth factor-β.

Original languageEnglish (US)
Pages (from-to)2457-2463
Number of pages7
JournalEndocrinology
Volume137
Issue number6
DOIs
StatePublished - 1996

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Osteoclasts
T-Lymphocytes
Transforming Growth Factors
Coculture Techniques
Interleukin-4
Bone Marrow
Immunomagnetic Separation
CD8 Antigens
In Vitro Techniques
CD4 Antigens
Lymphocyte Subsets
Neutralizing Antibodies
Bone Marrow Cells
Chronic Disease
Cytokines
Bone and Bones
Cell Line

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

John, V., Hock, J. M., Short, L. L., Glasebrook, A. L., & Galvin, R. J. S. (1996). A role for CD8+ T lymphocytes in osteoclast differentiation in vitro. Endocrinology, 137(6), 2457-2463. https://doi.org/10.1210/en.137.6.2457

A role for CD8+ T lymphocytes in osteoclast differentiation in vitro. / John, Vanchit; Hock, Janet M.; Short, Lorri L.; Glasebrook, Andrew L.; Galvin, Rachelle J Sells.

In: Endocrinology, Vol. 137, No. 6, 1996, p. 2457-2463.

Research output: Contribution to journalArticle

John, V, Hock, JM, Short, LL, Glasebrook, AL & Galvin, RJS 1996, 'A role for CD8+ T lymphocytes in osteoclast differentiation in vitro', Endocrinology, vol. 137, no. 6, pp. 2457-2463. https://doi.org/10.1210/en.137.6.2457
John, Vanchit ; Hock, Janet M. ; Short, Lorri L. ; Glasebrook, Andrew L. ; Galvin, Rachelle J Sells. / A role for CD8+ T lymphocytes in osteoclast differentiation in vitro. In: Endocrinology. 1996 ; Vol. 137, No. 6. pp. 2457-2463.
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