A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes

Cataldo Schietroma, Hoi Ying Yu, Mark Wagner, Joy A. Umbach, William M. Bement, Cameron B. Gundersen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Xenopus oocytes undergo dynamic structural changes during maturation and fertilization. Among these, cortical granule exocytosis and compensatory endocytosis provide effective models to study membrane trafficking. This study documents an important role for myosin1e in cortical granule exocytosis. Myosin1e is expressed at the earliest stage that cortical granule exocytosis can be detected in oocytes. Prior to exocytosis, myosin1e relocates to the surface of cortical granules. Overexpression of myosin1e augments the kinetics of cortical granule exocytosis, whereas tail-derived fragments of myosin1e inhibit this secretory event (but not constitutive exocytosis). Finally, intracellular injection of myosin1e antibody inhibits cortical granule exocytosis. Further experiments identified cysteine string proteins as interacting partners for myosin1e. As constituents of the membrane of cortical granules, cysteine string proteins are also essential for cortical granule exocytosis. Future investigation of the link between myosin1e and cysteine string proteins should help to clarify basic mechanisms of regulated exocytosis.

Original languageEnglish
Pages (from-to)29504-29513
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number40
DOIs
StatePublished - Oct 5 2007

Fingerprint

Exocytosis
Myosins
Xenopus
Oocytes
Membranes
Structural dynamics
Kinetics
Antibodies
cysteine string protein
Experiments
Endocytosis
Fertilization
Tail
Injections

ASJC Scopus subject areas

  • Biochemistry

Cite this

Schietroma, C., Yu, H. Y., Wagner, M., Umbach, J. A., Bement, W. M., & Gundersen, C. B. (2007). A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes. Journal of Biological Chemistry, 282(40), 29504-29513. https://doi.org/10.1074/jbc.M705825200

A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes. / Schietroma, Cataldo; Yu, Hoi Ying; Wagner, Mark; Umbach, Joy A.; Bement, William M.; Gundersen, Cameron B.

In: Journal of Biological Chemistry, Vol. 282, No. 40, 05.10.2007, p. 29504-29513.

Research output: Contribution to journalArticle

Schietroma, C, Yu, HY, Wagner, M, Umbach, JA, Bement, WM & Gundersen, CB 2007, 'A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes', Journal of Biological Chemistry, vol. 282, no. 40, pp. 29504-29513. https://doi.org/10.1074/jbc.M705825200
Schietroma, Cataldo ; Yu, Hoi Ying ; Wagner, Mark ; Umbach, Joy A. ; Bement, William M. ; Gundersen, Cameron B. / A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 40. pp. 29504-29513.
@article{d2a355489b554983b60a2609c75cde58,
title = "A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes",
abstract = "Xenopus oocytes undergo dynamic structural changes during maturation and fertilization. Among these, cortical granule exocytosis and compensatory endocytosis provide effective models to study membrane trafficking. This study documents an important role for myosin1e in cortical granule exocytosis. Myosin1e is expressed at the earliest stage that cortical granule exocytosis can be detected in oocytes. Prior to exocytosis, myosin1e relocates to the surface of cortical granules. Overexpression of myosin1e augments the kinetics of cortical granule exocytosis, whereas tail-derived fragments of myosin1e inhibit this secretory event (but not constitutive exocytosis). Finally, intracellular injection of myosin1e antibody inhibits cortical granule exocytosis. Further experiments identified cysteine string proteins as interacting partners for myosin1e. As constituents of the membrane of cortical granules, cysteine string proteins are also essential for cortical granule exocytosis. Future investigation of the link between myosin1e and cysteine string proteins should help to clarify basic mechanisms of regulated exocytosis.",
author = "Cataldo Schietroma and Yu, {Hoi Ying} and Mark Wagner and Umbach, {Joy A.} and Bement, {William M.} and Gundersen, {Cameron B.}",
year = "2007",
month = "10",
day = "5",
doi = "10.1074/jbc.M705825200",
language = "English",
volume = "282",
pages = "29504--29513",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "40",

}

TY - JOUR

T1 - A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes

AU - Schietroma, Cataldo

AU - Yu, Hoi Ying

AU - Wagner, Mark

AU - Umbach, Joy A.

AU - Bement, William M.

AU - Gundersen, Cameron B.

PY - 2007/10/5

Y1 - 2007/10/5

N2 - Xenopus oocytes undergo dynamic structural changes during maturation and fertilization. Among these, cortical granule exocytosis and compensatory endocytosis provide effective models to study membrane trafficking. This study documents an important role for myosin1e in cortical granule exocytosis. Myosin1e is expressed at the earliest stage that cortical granule exocytosis can be detected in oocytes. Prior to exocytosis, myosin1e relocates to the surface of cortical granules. Overexpression of myosin1e augments the kinetics of cortical granule exocytosis, whereas tail-derived fragments of myosin1e inhibit this secretory event (but not constitutive exocytosis). Finally, intracellular injection of myosin1e antibody inhibits cortical granule exocytosis. Further experiments identified cysteine string proteins as interacting partners for myosin1e. As constituents of the membrane of cortical granules, cysteine string proteins are also essential for cortical granule exocytosis. Future investigation of the link between myosin1e and cysteine string proteins should help to clarify basic mechanisms of regulated exocytosis.

AB - Xenopus oocytes undergo dynamic structural changes during maturation and fertilization. Among these, cortical granule exocytosis and compensatory endocytosis provide effective models to study membrane trafficking. This study documents an important role for myosin1e in cortical granule exocytosis. Myosin1e is expressed at the earliest stage that cortical granule exocytosis can be detected in oocytes. Prior to exocytosis, myosin1e relocates to the surface of cortical granules. Overexpression of myosin1e augments the kinetics of cortical granule exocytosis, whereas tail-derived fragments of myosin1e inhibit this secretory event (but not constitutive exocytosis). Finally, intracellular injection of myosin1e antibody inhibits cortical granule exocytosis. Further experiments identified cysteine string proteins as interacting partners for myosin1e. As constituents of the membrane of cortical granules, cysteine string proteins are also essential for cortical granule exocytosis. Future investigation of the link between myosin1e and cysteine string proteins should help to clarify basic mechanisms of regulated exocytosis.

UR - http://www.scopus.com/inward/record.url?scp=35748932034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748932034&partnerID=8YFLogxK

U2 - 10.1074/jbc.M705825200

DO - 10.1074/jbc.M705825200

M3 - Article

VL - 282

SP - 29504

EP - 29513

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 40

ER -