A scanning electron microscopic study of secondary lamellae and chloride cells of rainbow trout (Salmo gairdneri)

Kenneth Olson, Paul O. Fromm

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Scanning electron micrographs of gill tissue from rainbow trout fixed with 50% glutaraldehyde revealed the presence of microridges on surfaces of epithelial cells of the secondary lamellae. These microridges vary in length from 1 to 7 μ, with a mean height of 0.75 μ. Calculations show that they increase the total lamellar epithelial surface area approximately 2.5 fold. Mucus secreting cells are present on the body of the filament and on secondary lamellae. Chloride cells are located primarily in the interlamellae filamental epithelium and on the basal area of lamellae. Extensions of the chloride cell epithelium are microvillous in nature and their height is only slightly greater than that of the microridges of typical lamellar epithelial cells. A reduction in number or complete absence of microvilli on chloride cells appeared to be related to degenerative changes in these cells observed in transmission electron micrographs. Non secretory interlamellae filamental epithelial cells have microridges of very attenuated lengths.

Original languageEnglish (US)
Pages (from-to)439-449
Number of pages11
JournalZeitschrift für Zellforschung und Mikroskopische Anatomie
Volume143
Issue number4
DOIs
StatePublished - Dec 1973
Externally publishedYes

Fingerprint

Oncorhynchus mykiss
Chlorides
Electrons
Scanning
Epithelial Cells
Glutaral
Epithelium
Tissue
Mucus
Microvilli

Keywords

  • Chloride cells
  • Gills
  • Lamellae
  • Trout
  • Ultrastructure

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology
  • Medicine(all)

Cite this

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abstract = "Scanning electron micrographs of gill tissue from rainbow trout fixed with 50{\%} glutaraldehyde revealed the presence of microridges on surfaces of epithelial cells of the secondary lamellae. These microridges vary in length from 1 to 7 μ, with a mean height of 0.75 μ. Calculations show that they increase the total lamellar epithelial surface area approximately 2.5 fold. Mucus secreting cells are present on the body of the filament and on secondary lamellae. Chloride cells are located primarily in the interlamellae filamental epithelium and on the basal area of lamellae. Extensions of the chloride cell epithelium are microvillous in nature and their height is only slightly greater than that of the microridges of typical lamellar epithelial cells. A reduction in number or complete absence of microvilli on chloride cells appeared to be related to degenerative changes in these cells observed in transmission electron micrographs. Non secretory interlamellae filamental epithelial cells have microridges of very attenuated lengths.",
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T1 - A scanning electron microscopic study of secondary lamellae and chloride cells of rainbow trout (Salmo gairdneri)

AU - Olson, Kenneth

AU - Fromm, Paul O.

PY - 1973/12

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N2 - Scanning electron micrographs of gill tissue from rainbow trout fixed with 50% glutaraldehyde revealed the presence of microridges on surfaces of epithelial cells of the secondary lamellae. These microridges vary in length from 1 to 7 μ, with a mean height of 0.75 μ. Calculations show that they increase the total lamellar epithelial surface area approximately 2.5 fold. Mucus secreting cells are present on the body of the filament and on secondary lamellae. Chloride cells are located primarily in the interlamellae filamental epithelium and on the basal area of lamellae. Extensions of the chloride cell epithelium are microvillous in nature and their height is only slightly greater than that of the microridges of typical lamellar epithelial cells. A reduction in number or complete absence of microvilli on chloride cells appeared to be related to degenerative changes in these cells observed in transmission electron micrographs. Non secretory interlamellae filamental epithelial cells have microridges of very attenuated lengths.

AB - Scanning electron micrographs of gill tissue from rainbow trout fixed with 50% glutaraldehyde revealed the presence of microridges on surfaces of epithelial cells of the secondary lamellae. These microridges vary in length from 1 to 7 μ, with a mean height of 0.75 μ. Calculations show that they increase the total lamellar epithelial surface area approximately 2.5 fold. Mucus secreting cells are present on the body of the filament and on secondary lamellae. Chloride cells are located primarily in the interlamellae filamental epithelium and on the basal area of lamellae. Extensions of the chloride cell epithelium are microvillous in nature and their height is only slightly greater than that of the microridges of typical lamellar epithelial cells. A reduction in number or complete absence of microvilli on chloride cells appeared to be related to degenerative changes in these cells observed in transmission electron micrographs. Non secretory interlamellae filamental epithelial cells have microridges of very attenuated lengths.

KW - Chloride cells

KW - Gills

KW - Lamellae

KW - Trout

KW - Ultrastructure

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